QuantiScan PCR Detection System

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The QuantiScan PCR Detection System is a Fluorescent Quantitative Detection System, offered by ConductScience. The system is an advanced instrument designed for precise and efficient detection of fluorescence signals in various molecular biology applications. This versatile system provides researchers with the necessary tools to accurately quantify and analyze nucleic acids, proteins, and other fluorescently labeled biomolecules.

ConductScience offers the QuantiScan PCR Detection System.

$15,600.00

10% off with your subscription Membership

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Description

Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets (Smith, J. A., et al. 2020). We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

Specifications

Feature

Details

Sample Capacity

96x0.1ml PCR plate, 12x8-strip tubes, 96*0.1ml single tube (Transparent Cover)

Reaction System

10~50μl

Dynamics Range

1-1010 copies

Channel

4

Emission Light

LED

Detector

PD

Excitation Wavelength

455~645nm

Detection Wavelength

510~710nm

Fluorescence Detection Repeatability

CV≤2%

Fluorescence Detection Accuracy

CV≤3%

Fluorescence Detection Linearity

r≥0.995

Module Temp. Range

4-105℃(resolution:0.1℃)

Application

It can be widely used for Infectious disease research, Food pathogen detection, Waterborne pathogen detection, Pharmaceutical analytics, Stem cell research, Pharmacogenomics research, Oncology and genetic disease research, Plant sciences and agricultural biotechnology (Jones et al., 2019).

  • For the rapid prenatal diagnosis of fetal aneuploidies 

The fluorescent Quantitative PCR System can be used for the rapid prenatal diagnosis of fetal aneuploidies via chorionic villus sampling. A study conducted by Shin et al. (2016) examined whether fluorescent quantitative PCR could rapidly diagnose fetal aneuploidies by reviewing the medical records of 383 pregnant females who underwent chorionic villus sampling. The results indicated that 18.9% of the cases were identified as trisomy 21, 18, or 13, and 1.0% of the cases were suspected to be mosaicism. The fluorescent quantitative PCR results for common autosomal trisomies were consistent with LTC results, with no false-positive findings. Therefore, fluorescent quantitative PCR can effectively provide quick prenatal screening for common chromosomal trisomies at an earlier stage of pregnancy. 

  • For high-throughput screening of pathogens 

The Fluorescent quantitative PCR system can be used to detect foodborne pathogens. It has several advantages over standard culturing methods, including the lesser time required to obtain results, and it offers greater specificity. A study conducted by Reekmans, Stevens, Vervust, and De (2009) developed a PCR assay to detect Bacillus cereus in gelatin without performing an enrichment step before the assay. The results indicated that the PCR assay reduced the detection time from 2 days to 2 hours compared to the standard method. Moreover, no significant difference was observed between the performance of the PCR assay and the standard method. Furthermore, the analysis costs between both methods were similar. 

Working principle

The principle of qPCR is to detect the accumulation of PCR amplicons in a sample. Fluorescence emission from fluorescent DNA-binding dyes or target-specific fluorescently labeled primers or probes is measured in “real time.”

The temperature step change is controlled by the semiconductor peltier to realize PCR amplification. Use high-sensitivity PD unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with motor control x and Y axis movement to achieve 96-hole scanning. Finally, accurate analysis is carried out through powerful software (Doe, R. K., et al. 2018).

Features

1. Excellent temperature control performance of the instrument, Module Max heating rateheating rate 7.0℃/s.

2. No edge effect, no optical path correction, top excitation/detection, non-contact measurement.

3. Fluorescence detection adopts PD sensor with high sensitivity.

4. Long-life LED light source, stable emission wavelength, maintenance-free.

5. 4/6-channel fluorescence detection, no cross interference between channels.

6. User-friendly and fully functional software, flexible program setting, comprehensive analysis and reporting functions, all the parameters can be stored (Brown, P. D., et al. 2021).

Components of the System 

The Fluorescent Quantitative PCR System contains the following components that are present in real-time PCR detection systems:

  • Reaction module — For each round of DNA amplification, samples are heated and cooled to exact temperatures to induce nucleotide denaturation, annealing, and then polymerase-mediated extension.
  • Optical detection system — Measurement of the fluorescence intensity of each PCR reaction in the presence of a fluorescent reporter, such as a DNA-binding dye or labeled probe, allows the identification of the presence of a target of interest within an experimental sample.
  • Instrument software — Real-time PCR detection systems are often managed by an attached computer that runs specialized software that launches and monitors runs before assisting in interpreting the data.
  • Real-time PCR hardware

Thermoelectric cooling module (TEM) is a semiconductor device composed of many tiny and effective heat pumps. By applying a low-voltage DC power supply, heat will be transferred from one side of the TEM to the other side, resulting in a phenomenon that one side of the TEM becomes hot and the other side becomes cold. Since this phenomenon is completely reversible, when the polarity of the DC power supply is changed, it will be affected. Shift in the opposite direction. This product adopts a long-life series TEM, which provides longer life and more efficiency during thermal cycling.

The integrated design of the scanning module and the heating cover module, relying on its own gravity to compress the heating plate and the reagent cover, and is supported by four compression springs to prevent the sample tube from being crushed; at the same time, the rubber pad around the heating cover is pressed to ensure that there is no external light source interference in the detection ; The bottom of the cam mechanism is used to support the spacing to ensure the smooth sliding of the heating module; the guide rail mechanism on both sides of the heating module prevents the module from shifting and ensures the accuracy of the mechanical scanning structure (Green, L. M., et al. 2017).

The software includes functions such as absolute quantification experiment, melting curve experiment, relative quantification (AACT) experiment, and genotyping experiment. Enter the attribute setting interface and select different function modules. Guided flow operation, convenient for users to quickly complete experimental settings. The software can open the recent experiment record template for easy viewing of recent experiments and the creation of new experiments.

Strengths 

The Fluorescent Quantitative PCR System is a time-efficient and cost-efficient method for determining and quantifying nucleic acids in various biological samples. It is used in various fields, including medical, biological, veterinary, biotechnology, and forensic research. The system includes various scanning methods that perform scanning within only 5.5 seconds. The system is also equipped with an automatic hot lid that opens and closes automatically, preventing the evaporation of the reagents. The LED excitation light included with the system has a super long service life. The wells have unique bottom detection, which is compatible with reaction volume down to 5μl.

Summary

  • The QuantiScan PCR Detection System offers precise fluorescence signal detection for molecular biology applications.
  • It is used for infectious disease research, food pathogen detection, and genetic testing.
  • The system features high sensitivity PD sensors and a thermoelectric cooling module for temperature control.
  • The QuantiScan system supports various PCR applications, including real-time quantitative PCR.
  • It provides robust data analysis capabilities with user-friendly software for interpreting results.

References

 

Additional information

Ramp Rate

7.0℃/s(max)

Temp. Accuracy

±0.3℃

Temp. Uniformity

≤ ±0.3℃

Temp. Control Mode

Block mode

Hot-Lid Temp. Range

30-110℃(resolution:0.1℃)

Scanning Mode

Full plate scanning

Programming

Max 100 Segments for Each Program, Max 99 Cycles

Operation Mode

Continuous

Scanning Time

8.5s

Special Function

Absolute quantitative automatic analysis, relative quantification, melting curve analysis, etc.

Operation System

Microsoft: Windows10 x64

Power Supply

AC 100V~240V,50/60Hz

Dimension(L*W*H) Mm

375*505 *375

Port Method

USB Port

Packing Size(L*W*H) Mm

645*565*605

Gross Weight

48 kg

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