spectrophotometry

Lab Basics

Fluorescence Spectrophotometry: Principles and Applications

Fluorescence spectrophotometry is a set of techniques that deals with the measurement of fluorescence emitted by substances when exposed to ultraviolet, visible, or other electromagnetic radiation. It has wide application in chemical and biological sciences as it can be used to analyze a biological system, by studying its interactions with fluorescent probe molecules (So and Dong, 2002, The International Pharmacopoeia,

November 28, 2019

Lab Basics

Nuclear Magnetic Resonance Spectroscopy

Two molecules may have the same number and type of atoms, but their properties would change depending on how they are arranged (i.e., the bonds linking them and their orientation). As an example, Ethanol and Dimethyl ether, both have one oxygen, two carbon, and six hydrogen atoms, but the structures and properties of both these compounds are different. Ethanol exists

November 21, 2019

Lab Basics

The Basics of Infrared Spectrophotometry

Spectroscopy refers to the interaction between electromagnetic (EM) radiation and matter as a function of its frequency or wavelength. From this, a number of quantitative and qualitative measurements defining aspects like sample concentrations, structure, chemical compositions etc, can be obtained depending on the spectroscopic technique and spectrum of EM radiation used. When EM radiation interacts with matter, its frequency can

October 10, 2019

Fluorescence Spectrophotometry: Principles and Applications

Nuclear Magnetic Resonance Spectroscopy

The Basics of Infrared Spectrophotometry

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