When tracking animal behavior, video quality is integral to accurate results.
When tracking animal behavior, video quality is integral to accurate results. However, researchers, specifically for those new to behavioral analysis and phenotypes, may not know where to start.
At Conduct Science, we have been helping researchers with their apparatus needs for over 10 years and know a thing or two about video tracking, especially since the launch of our own video tracking package ConductVision. Follow our tips below for optimal video tracking for the most accurate data analysis and results!
Color and Contrast
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- Apparatus Color: For the most accurate video tracking, you need to provide enough contrast between your subject and the maze. As a rule of thumb, contrast a light colored subject with a dark colored maze such as black, blue, or grey. A dark colored subject contrasts well against white, blue, or grey maze.
- See the image below as a good example: In dark/night conditions, a white floor contrasts well against the dark colored mouse.

If in doubt and for maximum versatility, a grey or blue maze is ideal to use with dark and light colored animals.
Ideal Camera and Footage Specifications
For accurate video tracking, you need a camera that will track fine details (resolution) at a speed to keep up with the subject’s movements (frame rate). We recommend our customers use a camera with specifications similar to the following at minimum:
- 1920*1080
- 2 million megapixels
- Frame rate: 60 fps
Our camera model features these specifications with a plug-and-play design, no drivers or software required. See for yourself below, all these videos were shot with a camera with the above specifications.
We recommend recording with a 16:9 aspect ratio.
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Camera Orientation And Mounting
Now that you’ve picked the right camera, the next step is mounting it above the maze properly. You need to ensure that the camera is mounted directly above the center of the maze with the entirety of the maze in the camera view.
To mount your camera, an adjustable ganty is a good choice to adjust the height of the camera above the maze as required and to ensure camera stability while shooting.
If your maze is a square or rectangle, align it perpendicular to the camera (see the image below as a good example).

It is very important to align your maze properly to the camera as when you need to track your footage, you’ll need to draw the tracking area in the software (see the image below as a good example).


Lighting and Reflections
Ideally, the maze used in your trials should be well-lit across the entire maze as necessary (with exceptions such as the Light Dark Box) with no lighting reflections, as this will affect the accuracy of video tracking.
We recommend using a diffuse lighting source, such as studio cameras or ring lights and placing them at the sides of the maze to avoid direct reflection of the lights from above.
As you can see in the image on the left of a Zebrafish Open Field in infrared (IR) conditions, it can be difficult to provide appropriate lighting when using a water-filled or clear maze.
It can take some adjustment to avoid reflections, some tips include:
- Using matte, opaque mazes only
- Using a backlight
Computer Specifications
While our software can run on a standard laptop, for optimal running of the software, we recommend using a laptop with an integrated graphics processing unit or (GPU). Essentially akin to a gaming laptop. We recommend the following specs:
- Windows 10 or 11
- 16 or 32GB RAM
- Discrete GPU
- 65Hz refresh rate
- CPU speed 2.4 GHz
- Processor: 4.7 GHz
- Hard drive: 1 TB SSD
- Graphics card RAM: 8 GB
- NVIDIA GeForce RTX 4060, 3070 or similar

Software Matters
There are many different options for video tracking packages on the market and it’s important to pick the right package for your needs and requirements. When choosing a software package, it’s important to consider:
- Software method: In the age of AI, machine learning methods allow for more accurate tracking packages. Conductvision automatically and accurately tracks key body points with 95%+ accuracy compared to other software use outdated drawing or contour-based methods to recognize body points.
- Frame rate: To capture quick and subtle behaviors, your software needs to utilize a high frame rate. Our software tracks at 30 frames per second, while other software tracks at 6-8 frames per second.
- Number of subjects: Some software packages cannot handle multiple subjects in social paradigms or Open Field/Novel Object paradigms. If they can, you have to mark each animal with an ear tag or colored marker. Conduct Vision tracks 4+ mice at once without tagging or marking them.
- Experience with video tracking software: For those with coding experience, an Open Source tracking package may be useful, but for those wanting an easy and ready to-use software package, a full package such as ConductVision includes all the maze paradigms you need, just upload or stream you footage into the program! We’re here 24/7 to support you.
- Price: Video tracking software can vary widely in price with different subscription options. ConductVision offers 3 different, competitively priced packages. If that doesn’t work, just ask! We’ll supply the right package at the right price.
Author

Louise Corscadden, PhD
Dr Louise Corscadden acts as Conduct Science’s Director of Science and Development and Academic Technology Transfer. Her background is in genetics, microbiology, neuroscience, and climate chemistry.