Zebrafish Light/Dark Maze

The Zebrafish Light/Dark Test is a valuable assay for studying anxiety and exploratory behavior in zebrafish. During the trial, zebrafish are placed in a tank divided into light and dark zones, and their preference for these areas is recorded. A higher tendency to remain in the dark area is often interpreted as an indicator of anxiety, while more time spent in the light area suggests lower anxiety levels. The test capitalizes on the zebrafish’s natural tendency to explore novel environments, providing insights into their emotional responses. ConductVision software can track the fish’s movements and generate detailed data on the time spent in each zone, offering a clear picture of the animal’s anxiety levels and behavioral tendencies.