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Rodent Hot/Cold Plate Pain Assay

See more by: MazeEngineers

$4,990.00

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Description

The Hot/Cold Plate Test is a widely recognized method for assessing thermal pain in rodents and is commonly used to evaluate the effectiveness of analgesic treatments. This test involves placing a mouse or rat in a chamber with a temperature-controlled floor surrounded by transparent acrylic walls. Observations focus on two main behaviors: paw licking and jumping.

The apparatus for the Hot/Cold Plate Test consists of a transparent glass cylinder and a temperature-regulated metal plate. The glass cylinder (20 cm in diameter and 25 cm in height) confines the rodent to the metal plate while allowing clear observation. The metal floor can be heated or cooled to various temperatures as needed for the experiment.

This device integrates both hot and cold stimuli in one unit. The cold plate component helps evaluate the animal’s sensitivity to cold, facilitating the study of cold-induced hyperalgesia and allodynia. This setup also enables the assessment of pain responses to unilateral cold stimuli, which is not achievable with the hot plate alone. The adjustable temperature controls provide the ability to precisely observe the animal’s nocifensive responses to different thermal stimuli, making it a valuable tool for testing analgesic effectiveness.

MazeEngineers provide the Hot/Cold Plate Test apparatus.

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Producer: MazeEngineers

MazeEngineers offers custom-built behavioral mazes at no extra cost—designed to fit your exact research needs. Eliminate reproducibility issues from poor sizing or lingering scent cues with precision-engineered, modular, and smart mazes that adapt in real time to animal behavior. Publish new protocols, run adaptive experiments, and push the boundaries of behavioral science.

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Description

Hot Cold Plate Features

Acrylic Wall: Diameter 20 cm Ɨ 25 cm

Volume: Depth: 35 cm Ɨ Width: 30 cm Ɨ Height: 23 cm

Temperature setting range: -5ā„ƒ to 65ā„ƒ with 0.01ā„ƒ precision

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Features

Specifications
  • Temperature setting range: -10°C to 80°C, adjustment step 0.1°C
  • Temperature control error: <±0.2°C
  • Time control mode: panel buttons
  • Time display: 0.01 seconds to 1999.99 seconds
  • Timing error: 0.01 seconds
Data Excellence
  • Warming time: Approx 2 min (0°C to 55°C at 20° C)
  • Cooling time: 5 minutes ( 20 ā„ƒ to 0 ā„ƒ, or 0 ā„ƒ to -10 ā„ƒ)

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Introduction

The Hot/Cold Plate Test combines elements of both the Hot Plate and Cold Plate tests, which are typically conducted separately using distinct devices. This integrated test subjects animals to either harmful hot temperatures or benign cold temperatures to trigger nocifensive reactions. The Cold Plate component is particularly useful for evaluating cold-induced hyperalgesia and allodynia, and for assessing unilateral pain sensitivity. By merging these two types of pain assessments into a single apparatus, researchers can conveniently compare responses under both conditions and streamline their testing process.

The Hot/Cold Plate apparatus features a temperature-regulated surface that can be adjusted to maintain a constant temperature or to gradually increase or decrease in temperature. When exposed to these temperatures, animals may exhibit various nocifensive behaviors, such as jumping, paw licking, or stamping. Notably, hind paw responses like withdrawal and licking are often more reliable indicators of nociception compared to forepaw reactions, as the forepaws are typically engaged in exploration and grooming activities during the test.

Additionally, another temperature-based assay available is the Thermal Gradient Test.

Apparatus and Equipment

The Hot/Cold Plate apparatus features a transparent glass cylinder paired with a temperature-controlled metal plate. The metal floor of the plate is adjustable, allowing precise temperature regulation. The glass cylinder, with a diameter of 24 cm and a height of 29 cm, confines the subject to the plate, preventing it from escaping while ensuring a clear, unobstructed view for observation.

Training Protocol

Data Analysis

Data from the Hot/Cold Plate test is typically clear and direct. The primary measurement is the latency period, which is the time elapsed from when the subject is placed on the plate until the first indication of nocifensive behavior, such as paw licking or jumping. Additionally, other relevant data include the duration of the nocifensive response and the temperature at which such behaviors occur, especially in tests where the temperature is incrementally increased.

Strengths & Limitations

Summary & Key Points

  • Hot/Cold plate apparatus allows investigation of nocifensive responses to cold or hot temperatures within a single apparatus.
  • Hot/Cold Plate test is one of the many nociception tests available to test for pain response behaviors.
  • Hind paw withdrawal or licking is taken as the best indication of nocifensive response to thermal pain in hot plate test.
  • Hot/Cold Plate test is commonly used in evaluating the effectiveness of analgesics.
  • The cold plate allows assessment of cold-induced hyperalgesia and allodynia

Additional information

Weight 11 kg

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