Description
Hot Cold Plate Features | |
Acrylic Wall: Diameter 20 cm Ć 25 cm | |
Volume: Depth: 35 cm Ć Width: 30 cm Ć Height: 23 cm | |
Temperature setting range: -5ā to 65ā with 0.01ā precision |

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Features
- Temperature setting range: -10°C to 80°C, adjustment step 0.1°C
- Temperature control error: <±0.2°C
- Time control mode: panel buttons
- Time display: 0.01 seconds to 1999.99 seconds
- Timing error: 0.01 seconds
- Warming time: Approx 2 min (0°C to 55°C at 20° C)
- Cooling time: 5 minutes ( 20 ā to 0 ā, or 0 ā to -10 ā)
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Introduction
The Hot/Cold Plate Test combines elements of both the Hot Plate and Cold Plate tests, which are typically conducted separately using distinct devices. This integrated test subjects animals to either harmful hot temperatures or benign cold temperatures to trigger nocifensive reactions. The Cold Plate component is particularly useful for evaluating cold-induced hyperalgesia and allodynia, and for assessing unilateral pain sensitivity. By merging these two types of pain assessments into a single apparatus, researchers can conveniently compare responses under both conditions and streamline their testing process.
The Hot/Cold Plate apparatus features a temperature-regulated surface that can be adjusted to maintain a constant temperature or to gradually increase or decrease in temperature. When exposed to these temperatures, animals may exhibit various nocifensive behaviors, such as jumping, paw licking, or stamping. Notably, hind paw responses like withdrawal and licking are often more reliable indicators of nociception compared to forepaw reactions, as the forepaws are typically engaged in exploration and grooming activities during the test.
Additionally, another temperature-based assay available is the Thermal Gradient Test.
Apparatus and Equipment
The Hot/Cold Plate apparatus features a transparent glass cylinder paired with a temperature-controlled metal plate. The metal floor of the plate is adjustable, allowing precise temperature regulation. The glass cylinder, with a diameter of 24 cm and a height of 29 cm, confines the subject to the plate, preventing it from escaping while ensuring a clear, unobstructed view for observation.
Training Protocol
Data Analysis
Data from the Hot/Cold Plate test is typically clear and direct. The primary measurement is the latency period, which is the time elapsed from when the subject is placed on the plate until the first indication of nocifensive behavior, such as paw licking or jumping. Additionally, other relevant data include the duration of the nocifensive response and the temperature at which such behaviors occur, especially in tests where the temperature is incrementally increased.
Strengths & Limitations
Summary & Key Points
- Hot/Cold plate apparatus allows investigation of nocifensive responses to cold or hot temperatures within a single apparatus.
- Hot/Cold Plate test is one of the many nociception tests available to test for pain response behaviors.
- Hind paw withdrawal or licking is taken as the best indication of nocifensive response to thermal pain in hot plate test.
- Hot/Cold Plate test is commonly used in evaluating the effectiveness of analgesics.
- The cold plate allows assessment of cold-induced hyperalgesia and allodynia