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July 27, 2021

Classification and Biological Functions of Lipids

Introduction Lipids are one of the major macromolecules present in our body, and others include nucleic acids, carbohydrates, and proteins. But unlike the other macro biomolecules, lipids are not polymers - they aren’t composed of monomers. They are hydrophobic in nature because of the predominance of hydrocarbon chains (–CH2–CH2–CH2–CH2–) in…
July 19, 2021

Factors That Affects Enzyme Activity

Enzymes are a group of proteins that catalyze non-spontaneous chemical reactions in any biological system. In an organism, enzymes function as a group of interconnected chemical reactions in a metabolic pathway, fulfilling a specific cellular task. Metabolic pathways are active under normal circumstances and alter their activities in response to…
Molecular GeneticsScience
June 14, 2021

Cloning into Bacteriophage M13 Vectors

Insertion of foreign DNA into M13 cloning vectors is a bit tricky and includes the following steps: Ligating double-stranded DNA segments with cohesive termini into compatible sites in M13 double-stranded RF DNA. Transforming ligation products into competent male E. coli cells (plated in top agar supplemented with X-gal and IPTG. …