Science Archives - Conduct Science

April 13, 2021

A Brief on The NMR Spectroscopy of Proteins

Spectroscopy is “the study of investigation and measurement of the absorption and emission of light or electromagnetic radiation by matter as a function of its wavelength and frequency”. In simple terms, it’s the study of the interaction of light with a matter or substance. Depending on the nature of the…

Anjali Singh

Laboratory Techniques Protocols Science

April 5, 2021

The Nuclear Overhauser Effect

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated…

Anjali Singh

Laboratory Techniques Science

March 29, 2021

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Large-scale extraction of phage DNA involves digestion of viral coat proteins using a strong protease such as proteinase K followed by extraction with chloroform. This method is suitable for small-scale preparations as well. In the experiment, dialysis of bacteriophage suspension is followed by the extraction of phage particles with chloroform:…

Melissa Martinez

Laboratory Techniques Science

March 29, 2021

Electroporation-based CRISPR Cas9 delivery

CRISPR Cas9 is a potent and easy-to-use tool for gene editing, but the delivery of the CRISPR Cas9 system has been a challenge since it was developed. Several methods have been developed to effectively transport components of the CRISPR Cas9 system into the cells. The protocol below describes the electroporation-based…

Melissa Martinez

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

Laboratory Techniques Protocols Science

March 29, 2021

Polyacrylamide Gel Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a synthetic polymer that is considered the most all-around separation matrix to date due to its transparency, electrical neutrality, and adjustable pore size. The technique…

Arpaporn Sutipatanasomboon

Laboratory Techniques Science

March 29, 2021

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure is known as "preparation of λ arms." Restriction endonucleases are used to separate the two arms of phage DNA from the central stuffer fragment. Following…

Melissa Martinez

Laboratory Techniques Science

March 28, 2021

Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector

At each end of the central stuffer fragment, a series of restriction sites arranged in opposite directions are present in replacement vectors. For instance, in EMBL3A, the order of restriction site in the left polyclonal site is SalI-BamHI-EcoRI, and that of the right polyclonal site is EcoRI-BamHI-SalI. Digestion of this…

Melissa Martinez

Laboratory Techniques Science

March 26, 2021

Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA

Alkaline phosphatase treatment is used to suppress the background of non-recombinants while performing restriction digestion with insertion vectors containing a single cloning site or polyclonal site using a single restriction enzyme. In this method, ligated DNA is first subjected to phenol: chloroform extraction and standard ethanol precipitation, respectively. It is…

Melissa Martinez

Laboratory Techniques Protocols Science

March 15, 2021

Magnetic Resonance Imaging (MRI)

Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of successful events that started in 1946 when Felix Bolch proposed the new magnetic properties of the atomic nucleus. He explained the behavior of atoms at…

Anjali Singh

Laboratory Techniques Protocols Science

March 8, 2021

Introduction to Preparative Electrophoresis

Electrophoresis techniques have been primarily developed for analytical purposes— to investigate the characteristics of the molecules in the sample of interest. The techniques can be modified to serve a preparative purpose, in which a large amount of a specific molecule can be obtained at the end of an electrophoresis run.…

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Science

A Brief on The NMR Spectroscopy of Proteins

The Nuclear Overhauser Effect

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Electroporation-based CRISPR Cas9 delivery

Polyacrylamide Gel Electrophoresis

Purification of Bacteriophage λ Arms using Sucrose Density Gradients

Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector

Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA

Magnetic Resonance Imaging (MRI)

Introduction to Preparative Electrophoresis

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