$4,990.00
The Zebrafish Shuttlebox introduces a novel approach for conducting high-throughput experiments in learning paradigms. In this methodology, a pair of screens are employed within an automated computer interface to generate computer-animated images of zebrafish or any image designated by the researcher. The straightforward binary choice paradigm renders it a valuable tool for conducting high-throughput assays in zebrafish research.
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Features |
38 L experimental tank (50 Ć 25 Ć 30cm - width Ć depth Ć height) |
2.5 cm thin; grey; corrugated; plastic frame mounted to midline; side; and bottom of glass to minimize contact with video side of glass |
Price includes two monitors |
The Zebrafish Shuttle Box assessment serves as a tool for gauging the cognitive prowess of zebrafish. Employing a tank setup equipped with dual digital screens positioned at opposite ends, it delves into associative learning, temporal learning, and social behaviors exhibited by subjects in response to visual cues.
Zebrafish have emerged as prominent animal models owing to their genetic resemblance to humans and manageable upkeep requirements. Moreover, their strong social inclinations, favoring shoaling, contribute to the rationale behind the Zebrafish Shuttle Box’s design. Leveraging the fish’s social tendencies, subjects are inclined to approach and linger around animated depictions of conspecific groups, a phenomenon bolstered by the reinforcing properties of such visual stimuli (Al-Imari & Gerlai, 2008). By utilizing digital screens to present visual cues, researchers attain heightened control over spatial and temporal stimulus parameters, obviating the necessity for live conspecifics as visual aids.
Among other apparatuses tailored for zebrafish research are the Zebrafish Y-maze, the Zebrafish Three-Chamber Choice, and the Zebrafish Place Preference Test.
The Shuttle Box comprises a glass enclosure with a volumetric capacity of 38 liters. Its dimensions measure 50 cm in width, 25 cm in depth, and 30 cm in height. To facilitate experimental procedures, a narrow corrugated plastic frame, 2.5 cm in width, is affixed to the interior sides and bottom glass at the center of the tank. Positioned on the outer surface of the tank, monitors are symmetrically situated on opposing sides.
Ensure zebrafish are housed in tanks where temperature and pH remain stable. Consistently maintain environmental parameters to minimize stress and potential harm to the subjects. Oxygenate the water to uphold its quality at an optimal level. Prior to conducting tests, thoroughly clean the tank. Employ tracking systems like Noldus Ethovision XT to monitor and document fish behaviors. Notably, the Zebrafish Shuttle Box task does not necessitate habituation or pretraining.
The following parameters can be observed using the Zebrafish Shuttle Box.
Al-Imari, L., & Gerlai, R. (2008).Ā Sight of conspecifics as reward in associative learning in zebrafish (Danio rerio).Ā Behavioral Brain Research,Ā 189:216ā9
Pather, S., &Ā Gerlai, R., (2009)Ā Shuttle box learning in zebrafish (Danio rerio).Ā Behav Brain Res, 196(2), pp.323-7
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