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Mastering the Microtome
Laboratory Techniques

The Cryostat-Microtome

A Glimpse into the History of a Cryostat A Cryostat is a versatile and high-quality machine that generates low temperatures for tissue sectioning. The word “Cryostat” originates from two separate Greek words “Kryos”, meaning cold, and “stat”, meaning stable. Sir James Dewar, Scottish Physicist, and Chemist are credited for the use of the very first cryostats in the 19th century.
Spectrophotometer
Laboratory Techniques

A Brief on The NMR Spectroscopy of Proteins

Spectroscopy is “the study of investigation and measurement of the absorption and emission of light or electromagnetic radiation by matter as a function of its wavelength and frequency”. In simple terms, it’s the study of the interaction of light with a matter or substance. Depending on the nature of the interaction between the energy and material, spectroscopy is divided into
Spectrophotometer
Laboratory Techniques

The Nuclear Overhauser Effect

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated should be closer to the other nuclei, in space, to
SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory Techniques

Polyacrylamide Gel Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a synthetic polymer that is considered the most all-around separation matrix to date due to its transparency, electrical neutrality, and adjustable pore size. The technique can be applied to the analysis of proteins and nucleic
2 Magnetic resonance imaging (MRI)
Laboratory Techniques

Magnetic Resonance Imaging (MRI)

Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of successful events that started in 1946 when Felix Bolch proposed the new magnetic properties of the atomic nucleus. He explained the behavior of atoms at tiny magnets, and based on these, NMR tools were developed.
SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)
Laboratory Techniques

Introduction to Preparative Electrophoresis

Electrophoresis techniques have been primarily developed for analytical purposes— to investigate the characteristics of the molecules in the sample of interest. The techniques can be modified to serve a preparative purpose, in which a large amount of a specific molecule can be obtained at the end of an electrophoresis run. Preparative electrophoresis uses the same principles as analytical electrophoresis but