Global Data on Human Trafficking
Human Trafficking: Modern Slavery Exists The right to liberty is one of the essential human rights. Evidence, however, shows that slavery didn’t end with the Slavery Abolition Act in the 19th century. Modern slavery or human trafficking is a global problem affecting millions of people and societies across the world. In fact, data shows there […]
Making a First Impression with Grant Proposal
The first impression sets a tone, giving a brief but a vivid idea of your attitude and dedication towards a cause. Needless to say, everyone in the professional and academic field strives their best to make their first impression the best one. When it comes to proposals, making the first impression is perhaps the most […]
NIH Institutes and Centers (ICs) ā Who Does What
The National Institute of Health (NIH) is one of the oldest and the most prestigious health funding organizations in the U.S. Its objective is to bring betterment to human life through medical discoveries, quality healthcare services, and disease cures. It is a beacon of hope for millions of people battling health compilations and looking for […]
Zinc Staining Protocol
Introduction The reversible Zinc stain is a widely used staining method for the detection of proteins fractionated by polyacrylamide gels or SDS denatured gels. The Zinc reverse staining exploits the ability of biopolymers to bind Zn2+ and that of imidazole to react with unbound Zn2+ to produce insoluble zinc-imidazolate (ZnIm2).Ā This zinc-imidazole complex deposits along […]
NIH Pre-Award & Post-Award Process
The National Institute of Health (NIH) grant award is basically a formalization of a partnership between the NIH and the grant recipient to ensure abidance by laws, regulations, and policies. It protects the integrity as well as official and authorized acceptance of the overall endeavor. The NIH is the major federal funding agency that invests […]
Ponceau S Stain Protocol
Need stains for your experiments? Click here Introduction Ponceau S staining is a rapid and reversible staining method used for the detection of protein bands on Western blot membranes, Polyvinylidene fluoride (PVDF), nitrocellulose, and cellulose acetate membranes. Ponceau S is a negative stain that binds to the positively charged functional groups of the protein (amino […]
Pulse Chase Analysis
Need Incubators for your lab? Click here Ā As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Ā Introduction The pulse-chase analysis is a powerful technique to study the synthesis, processing, and transport of proteins. The pulse-chase analysis is widely used in biochemistry and molecular biology for the examination of the cellular process […]
Silver Staining Protocol
Need stains for your experiments? Click here Silver staining is a powerful technique for protein identification in gels as silver binds to chemical sidechains of the amino acids, including the carboxyl and sulfhydryl groups. It was introduced in 1972 and later adapted for protein separation from the polyacrylamide gel electrophoresis.Ā The nucleation sites in proteins […]
Southern Blotting Protocol
Need Laboratory Equipment for your reasearch? Click here Southern blotting is a hybridization technique used for deoxyribose nucleic acid (DNA). The method was named after Edward M. Southern, who developed the technique in the 1970s. The method involves the transfer of DNA fragments from an electrophoresis gel to a membrane causing immobilization of the DNA […]