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Luka Smilevski

The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Fluorescence Microscopy

Introduction Fluorescence microscopy is a specialized type of light microscopy that relies on the phenomena of fluorescence or phosphorescence. These terms describe materials that absorb light (photons) of one wavelength, then re-emit light of a second, longer wavelength.[1] The difference between fluorescence and phosphorescence is that the latter lasts much longer after the illumination is turned off. In fluorescence microscopy,
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Scanning Tunneling Microscope

Introduction Scanning Tunneling Microscopy, or STM, is a scanning probe technique. This means that the specimen is imaged by scanning a very sharp tip over its surface, while information about the shape or composition of the surface is collected based on interactions between the tip and the sample. This is unlike optical or electron microscopy, where a beam of light
The light digital microscope can be used to visualize structures as small as ~1 micron
Laboratory Techniques

Optical Microscopy: Specimen Preparation, Staining, and Quantitative Analysis

Introduction In most types of microscopy, the most complicated and sensitive aspect of the analysis is the preparation of specimens. The visualization of microorganisms using optical microscopy is no exception. Microorganisms are generally transparent, fragile, and highly sensitive to their environments, and for that reason, careful preparation is required to visualize them without significantly perturbing their structure. In this article,
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

The Light Microscope: Application Examples

Introduction The optical or light microscope is very well-suited for the imaging of biological specimens, for three main reasons: The resolution of the light microscope is close to or below the length scale of the structures of interest in biology. Microscopy does not require significant perturbation of the specimen. The versatility of the microscope allows for the development of highly