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Electromyography
Laboratory Techniques

Electromyography

Introduction Electromyography (EMG) is a specialized diagnostic technique used to study the muscle function by analyzing the electrical signals emanating from the nerves and the muscles. EMG can distinguish the abnormal or diseased muscle from the normal muscle. The electromyography can detect muscular abnormalities such as fasciculation or chronic denervation in clinically normal muscle. The neurogenic abnormalities detected by electromyography
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Total Internal Reflection Fluorescence Microscopy

As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Introduction Total internal reflection fluorescence microscopy (TIRFM) is a cutting-edge optical technique that excites the fluorophores in a fragile axial region to visualize the cellular events occurring close to the cell surface. The evanescent field, a near-field wave which decays in intensity over a sub-wavelength distance, is used as
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Scanning electron microscopy (SEM)

As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Introduction The scanning electron microscope employs a focused beam of high-energy electrons to visualize the regions of interest on the surface of solid specimens. The signals from electron-sample interactions unveil the information about the sample such as its morphology, chemical composition, and crystalline structure and material. The SEM can
Safranin Staining By Conductscience
Laboratory Techniques

Safranin Staining

Introduction Safranin is a cationic dye used in histology and cytology to distinguish and identify different tissues and cells.  It is popular in medical research for staining acidic proteoglycan that is found in cartilage tissues, enabling the researchers to analyze cell chondrogenesis. The safranin is employed as a counter-stain in endospore staining and Gram’s staining.  It is mostly utilized for
2 Magnetic resonance imaging (MRI)
Lab Basics

Magnetic resonance imaging (MRI) in Research

Introduction Magnetic resonance imaging (MRI) is an imaging technique used for the examination of anatomical structures, biological functions, and body fluids. The magnetic resonance imaging (MRI) uses the body’s natural magnetic properties to yield detailed images from any part of the body. For imaging, the hydrogen nucleus (a single proton) is used as it is abundant in water and fat.
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Fluorescence Resonance Energy Transfer (FRET) Microscopy

Introduction Fluorescence Resonance Energy Transfer (FRET) is a powerful physical phenomenon widely used in biomedical research and drug discovery. FRET technique involves the radiation-less transmission of energy from a fluorescent donor molecule to an acceptor molecule. The donor molecule is the chromophore or a dye that initially absorbs the energy, and the acceptor is the second chromophore to which the
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Fluorescence recovery after photobleaching (FRAP)

As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Introduction Fluorescence recovery after photobleaching (FRAP) is an imaging technique for the investigation of molecular dynamics and cellular events within living cells. The FRAP elucidates the dynamics and kinetics of the protein-protein interaction and cellular signaling. In a FRAP experiment, the laser beam is focused on a small area
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Electron Microscopy

As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Introduction Electron microscope is an imaging instrument that uses a beam of energetic electrons to observe objects on a very fine scale. Electron Microscopes were developed to overcome the limitations of light microscope which can only visualize the specimens with 500x or 1000x magnification and 0.2 micrometers resolution. In
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Transmission electron microscopy (TEM)

As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases Introduction Transmission electron microscopy (TEM) is an important tool in the expanding field of biomedical research and microbiology. The high resolution provided by the electron beams accelerated at high voltage make the TEM indispensable for detailed analysis. The specimen is an ultrathin section or a suspension on a grid.
The light digital microscope can be used to visualize structures as small as ~1 micron
Lab Basics

Electron Microscope

Electron microscopes are similar to light microscopes in many respects, but in place of light (photons), electrons are used to image the specimen. In a light microscope, the resolution is limited by diffraction, which becomes significant below the wavelength of visible light, 0.4 - 0.7 μm. In comparison, the wavelength of the electrons used in electron microscopy is less than