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SKU ME-8909 Category

Ant Sucrose Feeder 2

See more by: MazeEngineers

$990.00

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Sku: ME-8909 Category
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Description

The Ant Sucrose Feeder 2 is utilized to assess how ants collectively prefer paths based on distance and food source location.

This feeder features a diamond-shaped maze consisting of two V-shaped branches connected through their arms. The maze guides ants from the nest to the sucrose feeder containing the food source. Each branch is positioned at a 60-degree angle relative to the center axis to ensure minimal interference with the ants’ decision-making process. A short 2 cm section connects the longer branch to the shorter branch at both ends. The maze connects to the feeder and nest at opposite ends via a small 2 cm bridge.

This apparatus evaluates the subjects’ route memory by offering two paths of varying lengths that lead to the same food source.

Mazeengineers provides the Ant Sucrose Feeder 2 for research purposes.

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Description

Features

Length of V-shaped branches: 8cm

Angle of branches to the axis of the center:60o

Length of link between branches: 2cm

Length of link between maze and feeder: 2cm

Introduction

The ant sucrose feeder is employed to study how ants collectively prefer paths based on distance and food source accessibility. It assesses route memory by offering two paths of differing lengths to the same food source, shedding light on the mechanisms underlying ants’ decision-making processes. This device is valuable for creating various experimental setups involving memory and pheromones to test ants.

Pheromone deposition on the substrate significantly influences the collective decision-making of ants. However, individual behaviors and private information also impact group decisions. Combining private and social information in making composite path choices and organizing ants enhances collective decision-making effectiveness (T. Czaczkes, J. Czaczkes, Iglhaut, & Heinze, 2015). The design and specific treatments of the ant sucrose feeder challenge ants’ decision-making abilities in complex environments.

The ant sucrose feeder features a diamond-shaped maze that offers two distinct path choices for foraging. Decision points near the maze’s entrance and exit serve as critical decision-making junctures for the subjects. This design enables foragers to choose their paths while moving toward the feeder or returning to the nest. Other devices used to assess memory, learning, and decision-making in ants include the Ant Y Maze, Ant Double Bifurcation T, Ant T Maze, and Ant Binary Tree Maze.

Apparatus and Equipment

The ant sucrose feeder features a diamond-shaped maze composed of two V-shaped branches connected via their arms. Each side of the V-shaped branch measures 8 cm in length. This maze guides ants from their nest to the sucrose feeder containing the food source. Each branch is positioned at a 60-degree angle relative to the center axis to minimize any influence on ants’ decision-making caused by the branch orientation. A short 2 cm section connects the longer branch to the shorter branch at both ends. The maze connects to the feeder and nest at opposite ends through a small 2 cm bridge.

Training Protocol

To enhance subjects’ motivation for foraging, maintain them in a food-deprived state for 4 days before testing. Cleanse the apparatus with ethanol before and after each trial to eliminate any lingering pheromones. Utilize external tracking and recording systems like Noldus Ethovision XT to monitor and analyze the behavior of ants interacting with the ant sucrose feeder.

Position the diamond-shaped maze in proximity to the ant nest. Fill the feeder cavity with a 0.5 M sucrose solution. Initially, allow the ants to explore the maze with only the longer path open for 15 minutes. After this period, open the shorter path and observe the ants’ foraging behavior for the next 30 minutes as they access the food source using either path.

Czaczkes, Salmane, Klampfleuthner, and Heinze (2016) investigated the influence of route memory and pheromones on collective decision-making in ants during foraging. They used Lasius niger ants divided into eight colonies for their experiments. The ants were presented with the ant sucrose feeder, offering two distinct paths leading to the same food source (sucrose solution). The subjects utilized their memory and pheromone trails to distinguish between the shorter and longer paths.

The study included three treatments:

  1. In the ‘pheromone removed’ treatment, all apparatuses were cleaned with ethanol to eliminate previous pheromone trails.
  2. The ‘memory and pheromone remain’ treatment involved testing without cleaning the apparatus, using the same subject group.
  3. For the ‘memory removed’ treatment, a colony initially navigated the maze and was then replaced with a naive colony to follow the left pheromone trail.

 

Results indicated that in the ‘memory removed’ and ‘memory and pheromone remain’ treatments, a high proportion of ants chose the longer path through the maze to reach the food source, while the lowest proportion was observed in the ‘pheromone removed’ treatment.

Goss, Aron, Deneubourg, and Pasteels (1989) studied path choice behaviors in ants during foraging, focusing on Argentine Iridomyrmex humilis ants. The experiment utilized two diamond-shaped ant sucrose feeders, each offering a bi-dimensional path leading to the food source. Ants’ foraging activities were monitored as they navigated towards the food source and returned to the nest through the maze. Initially, an equal number of ants were observed in both arms of the maze. However, as the trail developed over time, a greater number of ants showed a preference for using the shorter arm to reach the food source.

Data Analysis

Following parameters are observed when using ant sucrose feeders

  • Number of ants at each feeder
  • Number of ants returning back
  • Number of ants switching feeder

Strengths and Limitations

The ant sucrose feeder is versatile for investigating various factors influencing foraging activity in ants. It facilitates the creation of diverse experimental setups by modifying memory and pheromone conditions. This straightforward device yields dependable results with minimal training necessary for experimentation. It is particularly useful for analyzing how ants choose paths based on path length and food source quality. The apparatus finds wide applications in exploring how route memory and pheromone concentrations influence ants’ decision-making abilities.

It’s crucial to maintain ants under consistent environmental conditions as during rearing. Variations in species, route memory, food sources, and foraging techniques can greatly influence task performance. The presence of olfactory or other disturbance cues can disrupt ants’ foraging behavior, underscoring the importance of thorough cleaning procedures.

Summary

  • The ant sucrose feeder can be used to study recruitment behavior, foraging activity, and memory acquisition in ants.
  • The apparatus is also used in investigations analyzing the effect of social and private information on ants’ decision process.
  • The diamond shape maze provides subjects with two different pathways leading to the same food source.

References

  1. Czaczkes, T. J., Czaczkes, B., Iglhaut, C., & Heinze, J. (2015). Composite collective decision-making. Proceedings of the Royal Society B: Biological Sciences, 282. doi:10.1098/rspb.2014.2723
  2. Czaczkes, T. J., Salmane, A. K., Klampfleuthner, F. A., & Heinze, J. (2016). Private information alone can trigger trapping of ant colonies in local feeding optimaThe Journal of Experimental Biology, 219(5), 744-51. doi:10.1242/jeb.131847
  3. Goss, S., Aron, S., Deneubourg, J.-L., & Pasteels, J. (1989). Self-organized Shortcuts in the Argentine Ant. Naturwissenschaften, 76(12), 579- 581. doi:10.1007/BF00462870

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