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Researcher exploring ANY-maze alternatives for rodent behavioral tracking in a modern laboratory setting

Top 5 Alternatives to ANY-maze for Behavioral Tracking (2025 Edition)

Behavioral Research Needs Are Evolving

ANY-maze has been a staple of behavioral tracking for years—helping labs automate simple video tracking for rodents across various mazes. However, as scientific demands evolve, researchers increasingly seek higher frame rates, markerless tracking, multi-animal tracking, and AI-driven precision to uncover deeper behavioral insights.

Today’s behavioral neuroscience labs need more than just zone entry data—they need real-time posture analysis, multi-species support, and batch processing power.

In this article, we’ll review the top alternatives to ANY-maze in 2025, with a focus on scalability, speed, flexibility, and next-gen technology.

#1. ConductVision (by Conduct Science)

The Most Advanced and User-Friendly Behavioral Tracking Solution

Why it’s #1: ConductVision redefines what’s possible in automated behavioral tracking:

  • 30+ frames per second high-speed tracking for real-time precision
  • Markerless tracking with automatic detection of 11 anatomical body points
  • Supports mice, rats, zebrafish, Drosophila, and birds—without any tags
  • Seamless tracking for social behavior, maze performance, locomotor assays, and multi-species studies
  • Batch video processing for rapid, high-throughput analysis
  • Intuitive user interface—no coding or complex setup required
  • Fully compatible with Conduct Science mazes (Barnes Maze, Elevated Plus Maze, Light/Dark Box, Open Field, and more)
  • Infrared and visible light camera support

Best for: Labs ready to upgrade to AI-powered, scalable tracking without sacrificing ease of use.

#2. ANY-maze (by Stoelting Co.)

Reliable but Becoming Outpaced by New Technologies

Strengths:

  • Easy setup and zone-based analysis
  • Good for single-animal, traditional maze tasks
  • Widely adopted in academic labs

Limitations:

  • Low frame rate (~6–8 fps standard)
  • Contour-based tracking (no body point detection)
  • Limited markerless multi-animal tracking
  • Basic locomotor outputs—lacks advanced posture/interaction data

Difficult to scale for high-throughput or multi-species studies

#3. EthoVision XT (by Noldus)

Powerful, but High Complexity and Cost

Strengths:

  • Widely used for maze and aquatic tracking
  • Good modularity (add-ons for social behavior, cognition, etc.)

Limitations:

  • Expensive to fully outfit for multi-paradigm studies
  • Requires complex calibration for multi-animal or small organisms
  • Less intuitive for new users

Best for: Larger, well-funded labs needing extensive modular control.

#4. DeepLabCut

Powerful Open-Source Pose Estimation with High Barriers to Entry

Strengths:

  • Deep learning-based body point tracking
  • Highly customizable for any species

Limitations:

  • Requires manual annotation of training sets
  • Coding and computational knowledge needed
  • Batch processing requires scripting

Best for: Computational neuroscience labs with machine learning expertise.

#5. ToxTrac

A Free, Lightweight Tool for Simple Locomotion Analysis

Strengths:

  • Open-source and free to use
  • Simple locomotion and zone tracking

Limitations:

  • Contour tracking only (no body point data)
  • No posture, social interaction, or aquatic model tracking
  • Limited support for complex paradigms or batch analysis

Best for: Labs on a tight budget or students starting basic behavior projects.

comparison table fo analyzing rodent behavior data using advanced tracking software

Final Thoughts: ConductVision—A True Step Forward

If you’re seeking the next generation of behavioral tracking, ConductVision delivers a rare combination of precision, versatility, and simplicity. With high-speed tracking, true markerless AI detection, and support for multiple species, it surpasses legacy systems like ANY-maze while remaining easy enough for any lab to adopt.

Ready to transform your behavioral research?
Learn more about ConductVision today

References:

  1. Stoelting Co. ANY-maze. https://www.anymaze.co.uk/
  2. Noldus Information Technology. EthoVision XT. https://www.noldus.com/ethovision-xt
  3. Mathis, A. et al. (2018). DeepLabCut. Nature Neuroscience.

Rodriguez, A. et al. (2018). ToxTrac. Scientific Reports.

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