Purification of Bacteriophage λ Arms using Sucrose Density Gradients
Introduction Replacement vectors or substitution vectors are prepared by removing the central filler segment of phage DNA to accommodate the fragments of foreign DNA. This procedure is known as the “preparation of λ arms.” Restriction endonucleases are used to separate the two arms of phage DNA from the central stuffer fragment. The following methods are […]
Preparation of Bacteriophage λ DNA Cleaved with two Restriction Enzymes to be used as a Cloning Vector
Introduction At each end of the central stuffer fragment, a series of restriction sites arranged in opposite directions are present in replacement vectors. For instance, in EMBL3A, the order of restriction site in the left polyclonal site is SalI-BamHI-EcoRI, and that of the right polyclonal site is EcoRI-BamHI-SalI. Digestion of this type of vector with […]
Alkaline Phosphatase Treatment of Bacteriophage λ Vector DNA
Introduction Alkaline phosphatase treatment is used to suppress the background of non-recombinants while performing restriction digestion with insertion vectors containing a single cloning site or polyclonal site using a single restriction enzyme. In this method, ligated DNA is first subjected to phenol: chloroform extraction and standard ethanol precipitation, respectively. It is then treated with restriction […]
CSP: Fossils
CSP – Fossils 00:00 – Intro 02:00 – News 11:05 – Factoids 16:15 – What are Fossils 26:00 – How fossils are formed 36:10 – Fossil fuels 39:30 – Burgess shale 46:20 – How fossils are used 52:40 – Giant penguins 57:40 – Questions 1:00:00 – Ending and outro You can listen to The Conduct […]
10 Networking Tips To Kickstart Your Graduate Career
The best tips to make your job hunting process easy-peasy A simple meaning of networking is communication. It’s something that we do in our daily lives, with new and existing friends, as well as family members. However, when it comes to “professional networking” – we want to grow connections for job and career advancement […]
CSP: Colour and Camouflage
CSP – Colour and Camouflage 00:00 – Introduction 01:21 – Announcement 03:15 – News 09:45 – Facts 12:45 – Animal and plant colorings 26:50 – Types of camouflage 37:15 – Crypsis 46:30 – Changing color 58:00 – Ending and outro You can listen to The Conduct Science Podcast by using the player above, searching for […]
Developing an Effective Competition-Based Approach to Changing Incentives in U.S. Healthcare
Name: Tim McDonald Number of lab members or colleagues (excluding PI): 10 Location: Pardee RAND Graduate School, University of Minnesota Graduation Date: Pardee RAND Ph.D. (expected 2021); Harvard Kennedy School M.P.P.; Hamline University B.A. Grants: Robert Wood Johnson Foundation (PI: Dowd),Donaghue Foundation (PI: Dowd); Horowitz Foundation for Social Policy Hello! Who are you and what […]
Magnetic Resonance Imaging (MRI)
Introduction Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of successful events that started in 1946 when Felix Bolch proposed the new magnetic properties of the atomic nucleus[1]. He explained the behavior of atoms at tiny magnets, and based […]
CSP: Dr. Lawrence Krauss – The Physics of Climate Change
CSP – Dr. Lawrence Krauss – The Physics of Climate Change 00:00 – Introduction 02:00 – Who is Dr. Krauss 13:00 – What is climate change 18:00 – The physics behind it 22:00 – How bad is it? 30:00 – CO2 36:00 – Other than CO2 38:00 – What can WE do individually 43:00 – […]