Description
Features |
Length of arms of maze: 20cm |
Width of arms of maze: 4cm |
Height of arms of maze: 4cm |
Angle between two choice arms: 1200 |

Introduction
The Ant Visual Discrimination Y-Maze is an important tool for examining visual discrimination in ants. These insects, which exhibit remarkable ecological diversity, inhabit a wide range of environments and employ various strategies like path integration and environmental cues for navigation (Schwarz & Cheng, 2010). In addition to pheromone trails, visual cues are vital for their navigation.
Visual discrimination tests, akin to the Visual Discrimination Chamber used for rodent studies, are widely utilized to assess visual learning and memory-related behaviors. The Ant Visual Discrimination Y-Maze offers a straightforward two-choice setup to evaluate foraging behaviors under different visual cues. This apparatus includes a long start arm that splits into two choice arms, forming a Y-Maze where cues can be placed within or at the ends of the choice arms. Research indicates that visual learning is influenced by the color, shape, and size of the cues, as well as the specific species and caste of the ants being studied (Fernandes, Buckley, & Nevin, 2017; Cammaerts, 2013). The Y-Maze is designed to accommodate large visual cues and other non-2D visual stimuli. Additionally, it can be used to investigate how the morphological variations in the eyes of different ant species impact visual perception and learning, as well as to evaluate choice behaviors.
Similar Y-mazes used for studying choice behaviors in various animals include the Honeybee Y-maze, the Bumblebee Y-maze, the Bat Y-maze, and the Zebrafish Y-maze.
Apparatus and Equipment
The Ant Visual Discrimination Y-Maze is designed in a Y shape, constructed from black opaque acrylic and mounted on a white Plexiglas plate. The maze features a straight start arm that divides into two choice arms. Each of the three arms measures 20 cm in length, 4 cm in width, and 4 cm in height. The choice arms are connected at a 120-degree angle at the decision point. The maze is equipped to hold both small-field and large-field visual stimuli. Small-field stimuli can be positioned 4.5 cm from the decision point, while large-field stimuli can be placed 20 cm away. The visual stimuli used in the maze include 2D patterns and LED lights.
Training Protocol
Clean the maze after every trial. Appropriately light the maze. A tracking and recording system such as the Noldus Ethovision XT can be used to assist with observations.
Literature Review
Data Analysis
The following can be observed on the Ant Visual Discrimination Y-Maze:
- Number of times the subject chose the rewarded visual stimulus
- Number of times the subject chose the punished visual stimulus
- Number of correct choices
- Number of errors
- Time spent in each arm
- Time taken to complete a trial
Strengths and Limitations
Summary
- The Ant Visual Discrimination Y-Maze is used to study visual discrimination in ants.
- It consists of a longitudinal start arm, and two choice arms joined together, forming a “Y” shaped apparatus.
- Different visual stimuli can be placed at the end of the choice arms that can vary in color, shape, size, or pattern.
- The subjects can easily be trained on the Ant Visual Discrimination Y-Maze, and trials can be conducted in a short amount of time.
References
- Aksoy, V., & Camlitepe, Y. (2012). Behavioural analysis of chromatic and achromatic vision in the ant Formica cunicularia (Hymenoptera: Formicidae). Vision research, 67, 28-36. doi:10.1016/j.visres.2012.06.013
- Cammaerts, M. C. (2013). Visual discrimination of shapes in the ant Myrmica rubra (Hymenoptera, Formicidae). Belgian journal of zoology, 143(1).
- Fernandes, A. S. D., Buckley, C. L., & Niven, J. E. (2017). Visual associative learning in wood ants. The Journal of Experimental Biology, 221(3), jeb173260.doi:10.1242/jeb.173260
- Schwarz, S., & Cheng, K. (2010). Visual associative learning in two desert ant species. Behavioral Ecology and Sociobiology, 64(12), 2033–2041. doi:10.1007/s00265-010-1016-y
- Yilmaz, A., Aksoy, V., Camlitepe, Y., & Giurfa, M. (2014). Eye structure, activity rhythms, and visually-driven behavior are tuned to visual niche in ants. Frontiers in behavioral neuroscience, 8, 205. doi: 10.3389/fnbeh.2014.00205
- Yilmaz, A., Dyer, A. G., Rössler, W., & Spaethe, J. (2017). Innate colour preference, individual learning and memory retention in the ant Camponotus blandus. Journal of Experimental Biology, 220(18), 3315-3326. doi:10.1242/jeb.158501