Protocols

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

Laboratory Techniques

Capillary Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Capillary electrophoresis is one type of electrophoresis in which the separation of molecules is performed in a capillary tube that is immersed in an electrophoresis running solution. It is the only type of electrophoresis that can be modified to be fully-automated and to accommodate all forms of electrophoresis, this

April 27, 2021

Laboratory Techniques

The Cryostat-Microtome

A Glimpse into the History of a Cryostat A Cryostat is a versatile and high-quality machine that generates low temperatures for tissue sectioning. The word “Cryostat” originates from two separate Greek words “Kryos”, meaning cold, and “stat”, meaning stable. Sir James Dewar, Scottish Physicist, and Chemist are credited for the use of the very first cryostats in the 19th century.

April 20, 2021

Laboratory Techniques

A Brief on The NMR Spectroscopy of Proteins

Spectroscopy is “the study of investigation and measurement of the absorption and emission of light or electromagnetic radiation by matter as a function of its wavelength and frequency”. In simple terms, it’s the study of the interaction of light with a matter or substance. Depending on the nature of the interaction between the energy and material, spectroscopy is divided into

April 13, 2021

Laboratory Techniques

The Nuclear Overhauser Effect

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated should be closer to the other nuclei, in space, to

April 5, 2021

Laboratory Techniques

Polyacrylamide Gel Electrophoresis

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a synthetic polymer that is considered the most all-around separation matrix to date due to its transparency, electrical neutrality, and adjustable pore size. The technique can be applied to the analysis of proteins and nucleic

March 29, 2021

Laboratory Techniques

Magnetic Resonance Imaging (MRI)

Magnetic resonance imaging is an advanced, non-invasive diagnostic medical imaging technique that provides three-dimensional images of organs. The discovery of MRI involves a series of successful events that started in 1946 when Felix Bolch proposed the new magnetic properties of the atomic nucleus. He explained the behavior of atoms at tiny magnets, and based on these, NMR tools were developed.

March 15, 2021

Laboratory Techniques

Introduction to Preparative Electrophoresis

Electrophoresis techniques have been primarily developed for analytical purposes— to investigate the characteristics of the molecules in the sample of interest. The techniques can be modified to serve a preparative purpose, in which a large amount of a specific molecule can be obtained at the end of an electrophoresis run. Preparative electrophoresis uses the same principles as analytical electrophoresis but

March 8, 2021

Laboratory Techniques

Cytogenetics: An advanced technique to color Chromosomes

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Cytogenetics is the study of the structural and functional properties of the chromosome. It includes chromosomal structure, function, behavior - during mitosis and meiosis, and influence on the phenotype of an organism. It is used as a tool to understand genetic phenomena due to chromosomal aberrations. It combines

April 20, 2020

Capillary Electrophoresis

The Cryostat-Microtome

A Brief on The NMR Spectroscopy of Proteins

The Nuclear Overhauser Effect

Polyacrylamide Gel Electrophoresis

Magnetic Resonance Imaging (MRI)

Introduction to Preparative Electrophoresis

Cytogenetics: An advanced technique to color Chromosomes

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