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A Brief on The NMR Spectroscopy of Proteins

Spectrophotometer

Spectroscopy is “the study of investigation and measurement of the absorption and emission of light or electromagnetic radiation by matter as a function of its wavelength and frequency”[8]. In simple terms, it’s the study of the interaction of light with a matter or substance. Depending on the nature of the interaction between the energy and […]

CSP: Parasites

CSP – Parasites 00:00 – Introduction 02:38 – News 10:26 – Facts 16:30 – What is parasitism 33:00 – Parasitic strategies 37:50 – Parasite examples 47:30 – Mind control parasites 53:00 – Alien: Xenomorphs 1:06:00 – Ending and outro You can listen to The Conduct Science Podcast by using the player above, searching for “The […]

Creating and Sustaining Movements in Medicine

Name: Alister Martin Number of core colleagues on your team: 7 Location: Cambridge, Massachusetts Graduation Date: 2015 H-index: 3 Grants: 3 Success of your lab’s members/colleagues: Shuhan is the Tony Stark of medicine. Twitter followers: 3500 Hello! Who are you and what are you working on? Hi! My name is Alister, I am an ER […]

Assessing Psychological Dynamics During the COVID-19 Pandemic

Name: Stephanie J. Eder Number of colleagues (excluding PI): 6 in the current project Location: Vienna, Austria Graduation Date: July 2020 Grants: 4 scholarships so far Twitter followers: 52 Hello! Who are you and what are you working on? I’m a trained psychologist – although I currently investigate C. elegans neuroscience. Together with colleagues from all over Europe, I recently published […]

The Nuclear Overhauser Effect

Spectrophotometer

Introduction The nuclear overhauser effect is defined as a change in the integrated intensity of one spin when the equilibrium population of another spin is perturbed by saturation or inversion. So, it is the transfer of nuclear spin polarization from one spin-active nucleus to another. However, the nuclei being irradiated should be closer to the […]

CSP: Entropy and Tenet

CSP – Entropy and Tenet 00:00 – Intro 05:15 – News 12:35 – What is Entropy 18:40 – Information entropy 25:40 – Exploring entropy 30:40 – the arrow of time 39:30 – Spoiler Warning 41:08 – Tenet Review 45:45 – The science of Tenet 58:30 – Questions of time 1:10:20 – Ending nad outro You […]

Large Scale Extraction of Bacteriophage λ DNA Using Proteinase K

Introduction and Principles Large-scale extraction of phage DNA involves digestion of viral coat proteins using a strong protease such as proteinase K followed by extraction with chloroform. This method is suitable for small-scale preparations as well.  In the experiment, dialysis of bacteriophage suspension is followed by the extraction of phage particles with chloroform: phenol, and […]

Electroporation-based CRISPR Cas9 delivery

CRISPR Cas9 is a potent and easy-to-use tool for gene editing, but the delivery of the CRISPR Cas9 system has been a challenge since it was developed. Several methods have been developed to effectively transport components of the CRISPR Cas9 system into the cells. The protocol below describes the electroporation-based delivery of Cas9 from Streptococcus […]

Polyacrylamide Gel Electrophoresis

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Polyacrylamide gel electrophoresis is one of the first forms of gel electrophoresis. Polyacrylamide is a synthetic polymer that is considered the most all-around separation matrix to date due to its transparency, electrical neutrality, and adjustable pore size. The technique can be applied to the […]