KJELDAHL Method Protocol

Introduction and Principle The Kjeldahl method was developed by a brewer called Johann Kjeldahl in 1883. The protocol is built on the principle that strong acid helps in the digestion of food so that it releases nitrogen which can be determined by a suitable titration technique. By observing the nitrogen concentration of the food, the […]

Protein Quantification Protocols

Introduction: Quantitative Method The estimation of proteins via the quantitative method is one of the basic requirements in biochemistry. Proteins, from various perspectives, are substantially more complex than nucleic acids. Thus, it has been hard to give laboratory protocols that can be applied to proteins. The precise quantitation of the amount of protein during the […]

SDS-Polyacrylamide Gel Electrophoresis at Neutral pH (NuPAGE)

Introduction and Principle The traditional Laemmli system denaturing conditions are stimulated by the Invitrogen NuPAGE SDS-PAGE gel system which is a revolutionary high-performance polyacrylamide gel electrophoresis system. NuPAGE gels use a unique buffer formulation to maintain a neutral operating pH during electrophoresis, which minimizes protein modifications that can result in poor band resolution. Gels are […]

Conduct Science Podcast: Climate Change

The ConductScience Podcast: Climate Change 00:00 – Intro 01:00 – Factoids 04:40 – Climate change denial 07:13 – What is climate change? 11:55 – Global water crisis 22:16 – Climate change and politics 27:50 – Individualistic views on climate change 32:05 – Geoengineering morality and methods 43:26 – Big companies going green 45:00 – Carbon […]

Nucleic Acid Quantification Protocols

Introduction: Quantitative Method DNA and RNA quantifications are broadly utilized in biological and biomedical research. Over the last ten years, many technologies have been developed to enable automated and high-throughput analyses. We are going to learn the assays and different methods of how DNA and RNA quantifications are carried out.   Introduction and Principle: SCHMIDT […]

Quantitative Phosphate Protocol

Introduction: Determination of Inorganic Phosphate in Biologic Samples To find out the inorganic phosphate in a protein-containing liquid biological sample an aqueous reagent comprises an acid. This acid is capable of reacting with the molybdate salt to form molybdic acid for complexation with phosphate to form phosphomolybdate complexes, a molybdate salt, a nonionic surfactant reagent […]

TRICINE-SDS-PAGE Protocol

Introduction and Principle Proteins in the mass range 1–100 kDa are commonly separated by Tricine–SDS-PAGE. For the resolution of proteins less than 30 kDa, it is the preferred electrophoretic system. As compared to other electrophoretic systems, the concentrations of acrylamide used in the gels are lower. Electroblotting is facilitated by these lower concentrations, which is […]

Western blotting Protocol

Introduction The Western blot is a biological technique that allows for the specific identification and characterization of proteins. In this technique, the proteins are assorted based on their molecular weight, and type, through gel electrophoresis. The results of the electrophoresis are then transferred to a polyvinylidene fluoride (PVDF) membrane producing a band for each protein. […]

ConductScience Podcast: Senses – Our Perception of Reality

The ConductScience Podcast: Senses – Our Perception of Reality 00:00 – Intro 00:49 – Our perception? 03:46 – Factoids 08:05 – How many senses do we have? 17:36 – Senses in the animal kingdom: electro reception and sharks 24:51 – Bioelectrogenesis 27:18 – Other senses 29:24 – Mantis shrimp 33:39 – Thermal detection 35:28 – […]