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Rodent Craniotomy Protocol
ProtocolsScience
September 13, 2019

Rodent Craniotomy Protocol

A craniotomy is a surgical procedure used to temporarily open a part of the skull to expose the brain for experimental manipulations. Research developments over the past two decades have made craniotomy safer, simpler, and more successful. In craniotomy, a bone flap is removed from the skull to reach the…
Conductscience
Controlled Cortical Impact for Traumatic Brain Injury
Protocols
September 13, 2019

Controlled Cortical Impact for Traumatic Brain Injury

Traumatic brain injury (TBI) is referred to as alteration or dysfunctionality in brain function, or brain pathology resulted by external trauma or force. TBI is a serious health condition because of its complexity and wide-reaching effects, including lesions, necrosis, and axon degeneration. Research on traumatic brain injury has been challenging…
Conductscience
Fluid Percussion Injury
Protocols
September 12, 2019

Fluid Percussion Injury

The fluid percussion injury (FPI) model is a widely used study model for traumatic brain injury. FPI model produces an injury through a craniectomy by applying a momentary fluid pressure pulse on to the exposed dura. This pulse is created by a pendulum striking to the piston of the fluid…
Conductscience
HuntingtonÔÇÖs-Disease-Rodent-Models
Protocols
September 12, 2019

Huntington’s Disease Rodent Models

Huntington’s disease (HD) is an inherited genetic neurological disorder characterized by cognitive dysfunction, abnormal body movements, and progressive cell death and neurodegeneration of the striatum and cerebral cortex. HD is a dominantly inherited disorder caused by a genetic mutation in the IT15 gene. This mutation is an abnormally expanded and…
Conductscience
ParkinsonÔÇÖs-Disease-Rat-Models
Protocols
September 12, 2019

Parkinson’s Disease Rat Models

Parkinson’s disease (PD) is a common neurodegenerative disease characterized by the loss of 50% to 70% of the dopaminergic neurons in the substantia nigra pars compacta (SNc), loss of dopamine (DA) in the striatum, and the development of intracytoplasmic inclusions called Lewy bodies (LB), composed of α-synuclein and ubiquitin. α-synuclein…
Conductscience
Laboratory TechniquesScience
July 5, 2019

Coomassie Brilliant Blue Stain Protocol

Coomassie brilliant blue (CBB) stain is a widely used method for routine visualization of proteins separated on polyacrylamide gels. It is an organic dye that makes complexes with basic amino acids, such as lysine, histidine, tyrosine, and arginine. The stain transfers an overall negative charge to the proteins allowing their…
Conductscience
Laboratory TechniquesScience
July 3, 2019

Protein Staining Methods

Protein separation and identification is critical for proteome analysis and requires high resolution and powerful protein characterization after gel electrophoresis. To identify the proteins in the gel, colorimetric and fluorescence staining techniques are used. The stains include anionic dyes (Coomassie brilliant blue), metal cations (imidazole-zinc), silver stain, fluorescent dyes, and…
Conductscience
Laboratory TechniquesScience
July 1, 2019

Zinc Staining Protocol

The reversible Zinc stain is a widely used staining method for the detection of proteins fractionated by polyacrylamide gels or SDS denatured gels. The Zinc reverse staining exploits the ability of biopolymers to bind Zn2+ and that of imidazole to react with unbound Zn2+ to produce insoluble zinc-imidazolate (ZnIm2). This…
Conductscience
Laboratory TechniquesProtocolsScience
June 30, 2019

Ponceau S Stain Protocol

Ponceau S staining is a rapid and reversible staining method used for the detection of protein bands on Western blot membranes, Polyvinylidene fluoride (PVDF), nitrocellulose, and cellulose acetate membranes. Ponceau S is a negative stain that binds to the positively charged functional groups of the protein (amino group) and the…
Conductscience
Laboratory TechniquesScience
June 30, 2019

Pulse Chase Analysis

As an Amazon Associate Conductscience Inc earns revenue from qualifying purchases The pulse-chase analysis is a powerful technique to study the synthesis, processing, and transport of proteins. The pulse-chase analysis is widely used in biochemistry and molecular biology for the examination of the cellular process by exposing the cells to…
Conductscience