Techniques - Conduct Science

Lab Basics Laboratory Techniques Science

March 2, 2020

Ultracentrifugation basics and applications

Ultracentrifugation is a specialized technique used to spin samples at exceptionally high speeds. Current ultracentrifuges can spin to as much as 150 000 rotations per minute (rpm) (equivalent to 1 000 000 g) (Biocompare, 2019b). However, extreme centrifugal forces may cause overheating, so to avoid sample damage, ultracentrifuges are equipped…

Adélia Mendes

Laboratory Techniques Science

February 24, 2020

Spin-Spin coupling in NMR

The structure of a molecule can be predicted using NMR spectroscopy. However, the interpretation of the signals in an NMR spectrum relies on several factors. One of the factors affecting the location of the peaks in an NMR spectrum is Chemical shift. The location of the peaks is important in…

Jaspreet Singh Grewal

Laboratory Techniques Science

February 17, 2020

Histochemical techniques to demonstrate Carbohydrates-Polysaccharides

Carbohydrates are the most abundant and one of the four essential macromolecules, required for the survival of living beings. Structurally, these are polyhydroxy aldehydes or ketones. Carbohydrates are divided into three classes depending upon the number of forming units (aldehyde or ketone), which are monosaccharides, oligosaccharides, and polysaccharides. These carbohydrates…

Anjali Singh

Laboratory Techniques

February 11, 2020

Histochemical demonstration of Acidic Compounds

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction The cell is composed of molecules of different chemical nature, whether they are acidic, basic, or neutral, all contribute to the functioning of the cell. Here, we will discuss the histochemical methods used to demonstrate the acidic components…

Anjali Singh

Lab Basics Laboratory Techniques

January 18, 2020

1H Nuclear Magnetic Resonance (NMR) Chemical shifts

NMR spectroscopy (more information here) is an ideal technique for identifying the structure of molecules. In order to elucidate the structure of a molecule using NMR spectroscopy, we require the sample itself, a reference molecule, and a deuterated organic solvent. This mixture is taken in a thin NMR tube and…

Jaspreet Singh Grewal

Laboratory Techniques Protocols

December 5, 2019

What is Autoradiography?

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Autoradiography is an imaging technique that uses radioactive sources contained within the exposed sample. In vitro autoradiography methods involve the isolation of cellular components such as DNA, RNA, proteins or lipids, followed by labeling with suitable radioisotopes. In in…

Conductscience

Lab Basics Laboratory Techniques

December 2, 2019

Histochemical Methods For Protein Demonstration

As an Amazon Associate, ConductScience Inc. earns revenue from qualifying purchases Introduction Proteins are macromolecules that perform a vital role in every functional aspect of life processes, like transportation of molecules, signaling, movement of cells, regulation of gene expression, etc. Their purification, localization, and distribution have always been a critical…

Conductscience

Laboratory Techniques Protocols

November 25, 2019

Histochemistry: Visualizing Cellular Chemistry

Histochemistry is comprised of two words Histo & Chemistry, which means the chemistry of tissues. In the year 1800, histochemistry became a part of science and now it is one of the most widely used techniques to help scientists localize and visualize cellular components, tissues, and other living structures. This…

Conductscience

Lab Basics Laboratory Techniques

November 21, 2019

Nuclear Magnetic Resonance Spectroscopy

Two molecules may have the same number and type of atoms, but their properties would change depending on how they are arranged (i.e., the bonds linking them and their orientation). As an example, Ethanol and Dimethyl ether, both have one oxygen, two carbon, and six hydrogen atoms, but the structures…

Conductscience

Protocols

November 8, 2019

Quinolinic acid rat HD model

Huntington’s disease (HD) is an inherited autosomal dominant neuropathological disease caused by a mutation in the huntingtin gene (htt). Preclinical animal models are necessary for the study of HD and the development of therapeutic interventions for the disease. Ideally, an animal model should demonstrate all the behavioral and neuropathological characteristics…

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Ultracentrifugation basics and applications

Spin-Spin coupling in NMR

Histochemical techniques to demonstrate Carbohydrates-Polysaccharides

Histochemical demonstration of Acidic Compounds

1H Nuclear Magnetic Resonance (NMR) Chemical shifts

What is Autoradiography?

Histochemical Methods For Protein Demonstration

Histochemistry: Visualizing Cellular Chemistry

Nuclear Magnetic Resonance Spectroscopy

Quinolinic acid rat HD model

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