Top 10 Health Funding Organizations
Need help with your Grant Application? Click here Biomedical research has evolved over the past few decades and, together with genetics, comprises one of the most investigated healthcare fields in the world. Biomedical advancement is not possible without research and development (R&D), which in turn, thrives upon funding, grants, and subsidy. A number of government, […]
What a Winning Grant Application Looks Like
What Is a Grant Application? A grant application is a justified document-of-need submitted to government/private institutions, corporations, foundations, or trusts for research funding. The grant, in the form of money, salaries, expenses, travel allowances, technical facilitation, and equipment ā supports the pool of emerging investigators or veteran biomedical scientists. Research funding serves as oxygen for […]
The Method Section – Using Citizen Science
The Method Section: Using Citizen Science 00:00 ā Introduction 01:09 ā Pros and cons of citizen science 04:58 ā Local citizen science 06:47 ā Creating a citizen science project and reducing poor data collection 12:29 ā Non-local citizen science 16:25 ā Key takeaways 18:14 ā Ending and outro Check out the three apps that I […]
Affinity Chromatography Protocol
Introduction Affinity chromatography is a versatile separation protocol that uses the biological interactions for characterization and detailed analysis of sample components. It is based on highly specific interactions between two molecules, such as the interactions between enzyme and its substrate, receptor, and ligand, or antibody and antigen. These reversible interactions are used for the purification […]
BRADFORD Protein Determination Protocol
Need Laboratory Equipment for your reasearch? Click here Introduction and Principle Developed by Marion M. Bradford in 1976, the Bradford assay is a quick protocol, and the same amount of proteins are used as the Lowry assay. This assay is somewhat accurate, and out-of-range samples can be retested within minutes. This assay is recommended for […]
Fast protein liquid chromatography (FPLC) Protocol
Introduction Fast protein liquid chromatography (FPLC) is a type of liquid chromatography that provides high resolution by small-diameter stationary phases for protein characterization and separation. The technique features high loading capacity, biocompatible buffer systems, fast flow rates, and stationary phases for common chromatography modes (e.g., gel filtration, ion exchange, reversed phase, and affinity chromatography). The […]
Gel Filtration Chromatography Protocol
Need benchtop equipment for your lab? Click here Introduction Gel-filtration chromatography, also known as size exclusion chromatography, is a versatile technique that permits the separation of proteins and other biological molecules. The gel filtration chromatography separates the proteins solely based on molecular size differences. For this, a porous matrix is used to which the molecules, […]
Ion-exchange Chromatography Protocol
Introduction Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a reversible interaction between charged molecules of the sample with charged ligands attached to a column. The method offers a sizeable sample-handling capacity, powerful resolving ability, broad applicability, moderate cost, and ease of handling. These characteristics […]
High performance liquid chromatography (HPLC) Protocol
Introduction High-performance liquid chromatography (HPLC) is a powerful separation technique used for the analysis of ions, proteins, and organic molecules. HPLC is based on the mechanisms of adsorption, partition, and ion exchange, which depend on the type of stationary phase used. The technique involves a solid stationary phase, usually enclosed in a stainless-steel column, and […]