The elevated plus maze test is one of the most widely used tests for measuring anxiety-like behavior. The test is based on the natural aversion of rodents to open and elevated areas, as well as on their natural spontaneous exploratory behavior in novel environments. This test is commonly used with therapeutic validation and screening for anti-anxiety properties. All open arms include 1cm high-end plates to ensure the mice do not fall off the maze during exploration.

Our second variation of the EPM is shaped as an elevated cross maze, which utilizes a clear central area with 4 door partitions.