Description
Specifications
Model | Semi-auto RF-RD-485 | Semi-auto PRO RF-RD-485PRO |
---|---|---|
Section thickness setting range: | 0-99.9µm increment: 0.1µm | |
Trimming thickness setting range: | 0-999µm increment: 0.1µm | |
Specimen retraction: | 0-100µm(can close) | |
Precision and error: | ±1% | |
Fast feed speed adjust: | 1mm/s ~3.5mm/s, at any set | |
Maximum specimen size: | 60mmx50mm | |
Maximum specimen size: | 28mm | |
Specimen vertical feeding: | 60mm | |
Slow feed speed adjust: | 0.1mm/s ~1mm/s, at any set | |
Specimen clamp rotation: | At any angle within 360° | |
Specimen orientation system | XY -15° | |
Net weight: | 35KG | |
Size: | 580mm*490mm*315mm(L*W*H) | |
Specimen clamp: | Paraffin block clamp or cassette clamp , optional | |
Blade holder: | In high or in low profile blade holder, optional | |
Voltage and frequency: | AC220V±10% 50Hz(standard model);AC110V±10% 60Hz |
Features
Standard Semi-auto Microtome_
RF-RD-485
PRO Semi-auto Microtome_
RF-RD-485PRO
Robust and Easy-to-Clean Design: Features an easy-to-clean housing and a sturdy base for stability. Includes a wide, removable waste tray.
Dual Handwheel Locking System: Offers two independent locking positions: at any 360° position and a quick lock at the top end.
Optimized Sample Feed: Provides continuous action from 0.1mm/s to 3.5mm/s and single-point action from 1µm to 100µm for sample forward and backward movement.
High-Definition Color Touch Screen: Displays all essential parameters like section mode, thickness, section counter, and retraction. Equipped with a high-precision controller and photoelectric sensing system for 0.1µm slicing accuracy.
Automatic Tool Alignment: Tissue automatically moves to the blade edge when activated, streamlining setup.
External Remote Control: Operate all functions conveniently via the included remote control.
Intelligent Slice Counting: Accurately records slicing data by automatically excluding trimming values.
Half-Cycle Reciprocal Mode: Features a specialized slicing/trimming mode for enhanced efficiency.
Configurable Sleep Mode: The machine can automatically enter a customizable sleep mode to save energy.
Triple Handwheel Locking: Enjoy three independent locking options: any 360° position, a quick top-end lock, and an electronic lock.
Adjustable Specimen Clamp: Offers 16° horizontal and vertical adjustment, plus 360° rotation for easy sample positioning during trimming.
One-Button Return: Quickly replace specimens with a simple press of a button.
Real-time Specimen Position Display: Monitor the clamp’s position in real time, complete with limit alarms.

Introduction
A Rotary microtome cuts the sections of biological specimens into thin slices for use in microscopy. It gets its name because of the rotary action of the handwheel used for slicing samples. It is used to prepare thin layers of bone, mineral, teeth, and hair with section thicknesses ranging from 1-micron to 60Ā microns. For hard materials that use synthetic resins, they can slice up to 0.5 microns.Ā
Parts of Rotary Microtome
- Knife Holder Base
The knife holder baseĀ holds the holder in place on the microtome stage. It can be moved to or away from the block, but it must remain fixed and secured during microtomy.
- Knife Holder
The knife holder comprises various parts, including the blade clamp, which holds the blade, the knife tilt, which adjusts the knife angle, and the faceplate, which directs the ribbons away from the blade and toward the operator.
- Microtome BodyĀ
The microtome body is a platform with rails that keeps the knife holder base in place.
- Cassette clamp or block holder
The block holder or cassette clamp holds the paraffin block in place. The block usually slides up and down with each revolution while the blade remains stationary. The block holder may be equipped with knobs that enable the operator to move the block face in different directions to line the tissue with the blade.
- Advancement Handwheel
The advancement handwheel rotates in one direction, moving the block closer to the knife at the preset microns.
- Coarse Handwheel
The coarse handwheel moves the block closer or away from the knife.
- Micron Adjustment
Micron adjustment is used to adjust settings for slice thickness that vary from 1 to 60 microns.
- Safety Lock
The handwheel includes a safety lock that prevents the wheel from loosening and the block holder from falling towards the blade when inserting or removing a block.Ā
Principle
The Rotary microtome uses a staged rotary action where the specimen is cut with the circular motion of the handwheel. The cutting procedure is done with a knife or disposable blade placed within the instrument.Ā
Protocol
- Embed the sample in a paraffin cartridge.
- Mount it on the paraffin block/sample holder.
- Set the distance between your knife blade and paraffin block using the coarse handwheel.
- Carefully install the blade and fix it in the knife holder.
- Set the required microns size by using the micron adjustment knob.
- Unlock the handle-equipped wheel by pushing the pin button-down located on its top.
- Revolve the handle-equipped wheel to operate the rotary microtome.
- Continue to cut until you get your desired sample.
Strengths and Limitations
The Rotary microtome is used to cut biological specimens according to the desired thickness ranging from 1 to 60 microns. It may be adapted to various types of tissue sectioning (hard, brittle, or fatty). However, it is not suitable for large tissue sectioning. Technological advancements in the rotary microtome have enhanced section quality, boosted production, and improved the technologist’s occupational safety.Ā
The incidence of repetitive motion disorders, a frequent occupational health concern in the histology laboratory, may be reduced by eliminating the manual hand-wheel operation of the microtome. Disposable blades are used, eliminating the need for sharpening and increasing sample size accuracy. Additionally, knives can be used in place of blades; they are thick and large and produce fewer vibrations during microtomy. A high-resolution motor makes a stable and smooth automatic slicing system. Its heavier size makes it stable, and it is crucial to avoid undulations in the paraffin sections. A double locking system makes it secure and prevents injuries during block handling. An emergency button is used to stop the operation in danger. It is anti-static, non-waxing, and is very easy to clean. It has four different automatic slicing modes that cover a lot of sampling situations. Daily cleaning from paraffin residue maintains a microtome cutting ideally for many years.
The heavy size of the rotary microtome makes it less portable. The disposable blades are expensive and need to be handled with care to avoid accidents. Moreover, the knives get dull if they aren’t used on decalcified tissue that hasn’t been cleaned.Ā
Summary
- The Rotary microtome uses a rotary motion to cut the sections of samples used in microscopy. It is used to prepare thin layers of bone, mineral, teeth, and hair with section thicknesses ranging from 1-micron to 60 microns.
- ItĀ uses a staged rotary action where the specimen is cut with the circular motion of the handwheel. The cutting procedure is done with a knife or disposable blade placed within the instrument.
- It has various parts: knife holder base, knife holder, microtome body,Ā cassette clamp or block holder, advancement handwheel, coarse handwheel, micron adjustment knob, and safety lock.
- It has thick and large knives that produce fewer vibrations. A high-resolution motor is used that creates a high-precision, stable, and smooth automatic slicing system.
- The rotary microtome is used in various microbiological labs, including histopathology/anatomical pathology labs.
- The blades of the rotary microtome should be handled with care. Always use cut-resistant gloves to remove the blades. Before cleaning, remove the blades from the holder and lock the rotary machine.
References
- Tosney, K. W., & Landmesser, L. T. (1986).Ā Neurites and growth cones in the chick embryo. Enhanced tissue preservation and visualization of HRP-labeled subpopulations in serial 25-microns plastic sections cut on a rotary microtome.Ā Journal of Histochemistry & Cytochemistry,Ā 34(7), 953ā957. https://doi.org/10.1177/34.7.3519758Ā
- Sharma, R. (2022). Microtomy. Retrieved February 5, 2022, from Slideshare.net website: https://www.slideshare.net/candysharma777/microtomy-67236029
- Frimiano, E M S, N, C. N., A, V. D., Sales, A., Mendes, & A, N. A. (2022). Histological study of the liver of the lizard Tropidurus torquatusWiedĀ 1820, (Squamata: Tropiduridae)(AU).Ā Braz. J. Morphol. Sci, 165ā170. Retrieved from https://pesquisa.bvsalud.org/portal/resource/pt/lil-644137
- Muhd-Farouk, H., Amin-Safwan, A., Mahsol, H., & Ikhwanuddin, M. (2017). Histological Characteristics on the Testes of Mud Spiny Lobster, Panulirus polyphagus (Herbst, 1793).Ā Pakistan Journal of Biological Sciences: PJBS,Ā 20(7), 365-371. https://doi.org/10.3923/pjbs.2017.365.371
- Sy, J., & Ang, L.-C. (2018).Ā Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded Sections.Ā Methods in Molecular Biology, 269ā278. https://doi.org/10.1007/978-1-4939-8935-5_23
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