The stereo or stereoscopic microscope, also known as the dissecting microscope, is a modification of the optical microscope intended for low magnification observation of an object, typically utilizing light reflected from the surface of an object as opposed to being transmitted through it. Stereomicroscopes work distinctively and address the needs of the users in a unique way. The stereo microscope offers a three-dimensional view of the sample, instead of a flat image. It also has a lower magnification normally ranging from 5x – 80x, but they provide a longer working distance.
Stereo microscopes have frequently nicknamed the workhorse of the lab or the production sector. Users spend numerous hours behind the ocular examining, viewing, reporting or dissecting specimen. These microscope devices are extremely versatile and are intended for observing whole objects, for example, rocks, insects, and flowers, yet can likewise be utilized for observing prepared slides. The three-dimensional feature makes the stereo microscope ideal for observing surfaces of solid objects. One can utilize these units for working with watches, circuits, and even microsurgery.
In general, the size of the stereomicroscope is larger than the size of a compound one, with the measurement of the former estimated to be approximately 1-2 feet in height. The design of the stereomicroscope varies according to the model. In some stereomicroscope systems, samples are imaged using two individual compound microscope optical trains, each comprising of an eyepiece, an objective, and intermediate lens components. Various other models utilize a typical objective shared between two separate optical channels. Two different images, instigating from somewhat distinct viewing angles, are projected onto the retinas of the user, where they excite nerve endings to transmit the data to the brain for processing. The outcome is a single three-dimensional image of the sample whose resolution is restricted by the optical framework parameters of the microscope and the number of nerve endings in the retina.
Stereo microscopes are essentially binocular which enable the utilization of stereoscopic vision. The stereomicroscope includes high numerical aperture objectives which can deliver high contrast images that have the lowest quantity of flare and geometrical distortion. The observation tubes have high eye-point eyepieces that can provide a field of view up to 26 millimeters. It also has a diopter adjustment that lets the image and reticle to merge into focus at the same time. Additionally, many units feature high zoom ratios (up to 12x-15x) that give a broad range of magnification (somewhere in the range of 2x and 540x) and decrease the need to change objectives. Ergonomic characteristics merged into the stereomicroscope designs help to decrease exhaustion during extended periods of operation, and new accessories allow present-day stereomicroscopes to image samples that were impractical only a couple of years back.
Stereomicroscopes can be generally divided into two fundamental types, each of which has both positive and negative attributes. The first type is the most established stereomicroscopic system, named after the creator Greenough, which uses twin body tubes that are inclined to generate the stereoscopic outcome. The Greenough design is exceptionally prominent and still utilized today by every important manufacturer. The framework utilizes two matching individual optical frameworks which are connected to the same stand at an angle. Two Porro prisms in the beam paths permit the image to be upright and accurately arranged. In addition, this design is comparatively inexpensive to manufacture. The second type is a newer framework known as the Common Main Objective (CMO). This system uses a single large objective that is shared by a pair of eyepiece tubes and lens. This version of stereo microscopes is still created today and is utilized for more sophisticated applications. This system takes into account modular use, and you can add various attachments, for example, a fluorescence attachment, iris diaphragm, ergonomic characteristics, illumination accessories, and so forth. Either type of microscope can be provided with step-type separate lenses to adjust magnification or a consistently variable zoom-type magnification system.
Selecting a microscope for using in a laboratory depends on the type of application and requirement of the user. For regular applications, for example, routine examination, PCB assessment, training, dissection, and so on, a Greenough style stereo microscope will be suitable. A Greenough model is more affordable and simple to utilize. For more sophisticated applications – for example, fluorescence or applications that entail higher resolution and magnification – a CMO type ought to be utilized. CMO microscopes will also enable the user to include ergonomic attributes into the magnifying instrument, for example, a tilting head.
Typically, a stereo microscope has three fundamental parts: a viewing head/body that contains the optical parts in the upper portion of the microscope, a focus block that connects the microscope head to the stand and helps the device to focus, and finally a stand that supports the microscope and accommodates any integrated lighting. The other components of a stereo microscope include eyepieces or oculars, eyepiece tubes, diopter adjustment ring, objective lenses, focus control, working stage, stage clips, and transmitted illumination.
The eyepieces are used for looking through the top of the microscope while the eyepiece tube holds the eyepieces in position over the objective lens. The diopter adjustment ring of the microscope enables the user to modify the focus on one eyepiece to balance for the disparity in vision between your two eyes. The objective lenses are the principal optical lenses on a microscope and provide fixed magnification or zoom magnification. The focus control allows the microscope to focus on the specimen. The working stage is where the sample to be viewed is positioned, and stage clips are used when the mechanical stage is not present. Transmitted illumination consists of a top light or a bottom light.
|View field diameter||20mm|
|0.7X-4.5X Objective lens ratio||1：6.4|
|Binocular Drawtube||Pupil Distance 55-77mm|
|Diopter 45°||Incline 360°Spin|
|View field||31.2 mm -5.1mm|
|Total Magnification||7X-45X (10XEyepiece), 3.5X-22.5X(10X Eyepiece +0.5X Assist lens)|
|Working Distance||100mm (10X Eyepiece)170mm (10X Eyepiece +0.5X Assist lens)|
|Package Items||Universal support(1 unit), Binocular drawtube (1 unit)，10X Eyepiece(1 pair), 0.5X Objective Lens (1 unit), Blinder (1 unit), Dust cover (1 unit), Instruction Manual (1 unit)|
- To begin using the stereomicroscope, place your microscope on a flat surface such as a tabletop which allows a lot of space to work.
- Connect the power cord of the instrument into a switch, ensuring that the excessive cord is out of the way.
- Now, turn on the transmitted illuminator (if a microscopic slide or other translucent object needs to be viewed, base lighting will be more suitable but if the specimen under observation is opaque or solid, the top lighting ought to be utilized so that the light can reflect off the object’s surface).
- Next, set the object on the stage plate.
- If the sample is thin and flat, try to utilize the stage clips to set its position by pulling up the pointed end of one stage clip and placing it on the end of the sample.
- Repeat the same process with the stage clip on the other side.
- Adjust the eyepiece(s) according to the proper interpupillary distance, so it is comfortable enough to view through the microscope without exerting strain on the eyes.
- To adjust the eyepiece, move the eyepieces closer together or farther apart until a single field of view is obtained.
- While glancing through the eyepiece, gradually spin the adjustment knob to the lowest power and allow the image to come into focus by utilizing the focus control.
- If you are unable to see anything, ensure that the specimen is placed directly below the objective lens and try again.
- Once the specimen has come into focus, move it around in order to view its different parts.
- Adjust the focus to a certain extent on each new region as a three-dimensional image being viewed may have a lot of different levels and it may not be possible to focus each characteristic clearly at the same time.
- Once the use of the microscope is finished, switch off the device and take out the sample.
- Remove the power cord and cover the microscope to protect from dust.
- Store the device in a place where it will not be damaged from severe hot or cool temperatures etc.