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Fully Automated Rotary Microtome Series

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Sku: RF-RD-RD-355AT /RD-355ATK Categories , , , Tags , ,
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Description

Conduct Science’s Automatic Rotary Microtome series, including the RF-RD-355AT (Standard Automatic) and RFRD-355ATK (Precision Automatic) models, offers advanced solutions for automated tissue sectioning. These instruments combine the efficiency of motorized operation with high-level precision, crucial for modern histology and pathology laboratories.

The RF-RD-355AT provides reliable, consistent sectioning for routine tasks, ensuring reproducible, high-quality sections. Stepping up, the RF-RD-355ATK is engineered for even greater accuracy and handling of larger specimens, making it ideal for more demanding research and diagnostic applications. Both models feature intelligent control systems and enhanced safety measures, streamlining workflow while maintaining operator safety and sample integrity. They represent a significant upgrade for environments seeking to improve throughput and consistency in tissue preparationConductScience offers the Semi-Auto Microtome Solutions

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Producer: Roundfin

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Description

Specifications

Model

Standard Automated Microtome

RF-RD-355AT 

Precision Automated Microtome

RF-RD-355ATK

Section thickness setting range:

0.5— 100um

0.5ļ½ž2 µm  increment 0.5 µm

2ļ½ž10 µm   increment 1 µm

10ļ½ž20 µm  increment 2 µm

20ļ½ž50 µm  increment 5 µm

50ļ½ž100 µm  increment 10 Āµm

0.25—100μm

             0.25—2.5μm   increment 0.25μm

             2.5—5μm     increment 0.5μm

             5.0—10μm    increment 1μm

             10—30μm    increment 2μm

             30—60μm    increment 5μm

             60—100μm   increment 10μm

Trimming thickness setting range:

0—500 Āµm

1—10 µm increment 1µm

10—20 µm increment 2 µm

20—100 Āµm increment 10 µm

100—500 µm increment 100 µm

1—600μm

               1—10μm      increment 1μm

               10—20μm   increment 2μm

               20—50μm   increment 5μm

               50—150μm   increment 10μm

               150—600μm   increment 50μm

Specimen feed speed

1250 Āµm/s  (click), 2000µm/s (keep pressing). (forward and reverse with 3 speed is optional customization,

3 different speed is (500µm/s or 1000µm/s or 2000µm/s)

1500μm/s ~ 3500μm/s

Cutting speed

0-300mm/s

(0-400 or 500mm/s is optional customization)

1500μm/s ~ 3500μm/s

Precision and error:

±5%

±1%

Specimen retraction setting range

0-220 µm

turn on or off is optional customization

0—50μm  ( 0 is off )

5-10-15—50 (optional)

Sample orientation

8°along the X-Y-axis, rotate 360°

8°along the X-Y-axis, rotate 360°

Sample size

Standard Embedding box Maximum 50*45mm

70Ɨ70mm (Larger capacity for bigger samples)

(optional clamp)

Horizontal specimen stroke

Standard 20mm,customization 28mm

Standard 20mm,customization 28mm

Vertical specimen stroke

Standard 60mm,customization 70mm

Standard 60mm,customization 70mm

Blade holder movement

Forward and backward:65mm

Left and right:±20mm

Forward and backward:65mm

Left and right:±20mm

Section base angle range

0-10°

0-10°

Blade height

99mm

99mm

Working temperature/Humidity

Working temperature:18ā„ƒļ½ž25ā„ƒļ¼›

/Humidityļ¼šā‰¤80%

Working temperature:18ā„ƒļ½ž25ā„ƒļ¼›

/Humidityļ¼šā‰¤80%

Working Atmospheric Pressure

860hpaļ½ž1060hpa

860hpaļ½ž1060hpa

Product size

(L) 500mm* (W)320mm* (H)500mm

580Ɨ 475Ɨ 340mm(WƗDƗH)

Features

Standard Automated Microtome RF-RD-355AT

Precision Automated Microtome RF-RD-355ATK

  • Intelligent Control System: Utilizes an intelligent feeding system that enables quick switching between manual or automated smart sectioning modes, with adjustable automatic slicing speed.

  • Advanced Drive System & Multifunctionality: Features advanced drive systems offering multiple functions, including sectioning, trimming, fast forward, fast backward, quick mode conversion, and automatic specimen retraction.

  • Enhanced Safety Measures: Incorporates a safety and emergency braking system, security alarm system, driver overload protection, and an auto-sleep protection system for secure operation. The handwheel balance is precisely adjusted and can be locked at any position to ensure slicing safety.

  • Optimized Blade Management: The blade holder can be moved laterally to utilize the entire length of the blade across three different segments, avoiding direct contact. A red protection bar covers the blade’s full length, and a push bar facilitates easy blade changes.

  • User-Friendly Interface: Equipped with an OLED screen that displays section and trimming thickness, as well as the number of sections. It also includes a remote control panel for easy operation.

  • Versatile Specimen Handling: Offers precise orientation for the specimen clamp and allows for easy and fast switching between different specimen clamps, with options for both paraffin block and cassette clamps. It also includes a large-volume, easy-to-remove waste tray where disposable blades can be stored

  • Intelligent Control System: Features an intelligent feeding system that allows for quick and easy switching between manual and automated smart sectioning modes, with adjustable automatic slicing speed for optimal control.

  • Advanced Drive & Multi-functional Operation: Equipped with advanced drive systems, this microtome offers multiple functions including precise sectioning, trimming, fast forward, fast backward, quick mode conversion, and automatic retraction for streamlined workflow.

  • Superior Sectioning Precision: Utilizes an international advanced screw motion mechanism to ensure exceptional precision and stable sectioning performance, consistently delivering high-quality slices.

  • Enhanced Safety Features: Designed with user safety in mind, it includes emergency braking systems, security alarms, driver overload protection, an auto-sleep protection system, and a balanced hand-wheel that can be locked in any position.

  • Versatile Blade Management: The blade holder can be moved laterally to maximize blade utilization across three segments without direct contact, and includes a red protection bar and push bar for safe and easy blade changes.

  • User-Friendly Design & Waste Management: Features a pure-green digital display for essential parameters, offers easy switching between specimen clamps, and includes a large-volume, easily removable waste tray with top storage for disposable blades.

Introduction

Manual rotary microtomesĀ are essential tools in various scientific and medical fields, particularly in histology, pathology, and material science, where the preparation of thin, uniform tissue sections is critical for microscopic examination. Our manual microtomes offer reliable performance, ease of use, and robust construction, making them indispensable for both routine laboratory work and more specialized research applications. This series is designed to provide consistent, high-quality sections, enabling accurate diagnosis, detailed research, and effective material analysis.

Parts of Rotary Microtome
  • Knife Holder Base

The knife holder baseĀ holds the holder in place on the microtome stage. It can be moved to or away from the block, but it must remain fixed and secured during microtomy.

  • Knife Holder

The knife holder comprises various parts, including the blade clamp, which holds the blade, the knife tilt, which adjusts the knife angle, and the faceplate, which directs the ribbons away from the blade and toward the operator.

  • Microtome BodyĀ 

The microtome body is a platform with rails that keeps the knife holder base in place.

  • Cassette clamp or block holder

The block holder or cassette clamp holds the paraffin block in place. The block usually slides up and down with each revolution while the blade remains stationary. The block holder may be equipped with knobs that enable the operator to move the block face in different directions to line the tissue with the blade.

  • Advancement Handwheel

The advancement handwheel rotates in one direction, moving the block closer to the knife at the preset microns.

  • Coarse Handwheel

The coarse handwheel moves the block closer or away from the knife.

  • Micron Adjustment

Micron adjustment is used to adjust settings for slice thickness that vary from 1 to 60 microns.

  • Safety Lock

The handwheel includes a safety lock that prevents the wheel from loosening and the block holder from falling towards the blade when inserting or removing a block.Ā 

Principle

The fundamental principle of all rotary microtomes, including our manual models, involves a precise mechanical action to achieve uniform tissue sections. A fixed tissue block, embedded in paraffin or resin, is mounted onto a specimen clamp. As the manual handwheel is rotated, a precisely engineered feeding mechanism advances the specimen block towards a stationary cutting blade. During each rotation, the specimen block moves past the blade, and a thin section of tissue is cleanly sliced off. The thickness of this section is determined by a highly accurate micrometer-based feed mechanism, which can be adjusted to increments as small as 1 micrometer. This continuous, controlled movement ensures that each section is of consistent thickness, critical for reproducible results in microscopy. Features like specimen retraction in models like the RD-315, RD-475, and RD-495 prevent the specimen from rubbing against the blade on the return stroke, extending blade life and improving section quality

Protocol

  1. Embed the sample in a paraffin cartridge.
  2. Mount it on the paraffin block/sample holder.
  3. Set the distance between your knife blade and paraffin block using the coarse handwheel.
  4. Carefully install the blade and fix it in the knife holder.
  5. Set the required microns size by using the micron adjustment knob.
  6. Unlock the handle-equipped wheel by pushing the pin button-down located on its top.
  7. Revolve the handle-equipped wheel to operate the rotary microtome.
  8. Continue to cut until you get your desired sample.

Strengths and Limitations

The Rotary microtome is used to cut biological specimens according to the desired thickness ranging from 1 to 60 microns. It may be adapted to various types of tissue sectioning (hard, brittle, or fatty). However, it is not suitable for large tissue sectioning. Technological advancements in the rotary microtome have enhanced section quality, boosted production, and improved the technologist’s occupational safety.Ā 

The incidence of repetitive motion disorders, a frequent occupational health concern in the histology laboratory, may be reduced by eliminating the manual hand-wheel operation of the microtome. Disposable blades are used, eliminating the need for sharpening and increasing sample size accuracy. Additionally, knives can be used in place of blades; they are thick and large and produce fewer vibrations during microtomy. A high-resolution motor makes a stable and smooth automatic slicing system. Its heavier size makes it stable, and it is crucial to avoid undulations in the paraffin sections. A double locking system makes it secure and prevents injuries during block handling. An emergency button is used to stop the operation in danger. It is anti-static, non-waxing, and is very easy to clean. It has four different automatic slicing modes that cover a lot of sampling situations. Daily cleaning from paraffin residue maintains a microtome cutting ideally for many years.

The heavy size of the rotary microtome makes it less portable. The disposable blades are expensive and need to be handled with care to avoid accidents. Moreover, the knives get dull if they aren’t used on decalcified tissue that hasn’t been cleaned.Ā 

Summary

  1. The Rotary microtome uses a rotary motion to cut the sections of samples used in microscopy. It is used to prepare thin layers of bone, mineral, teeth, and hair with section thicknesses ranging from 1-micron to 60 microns.
  2. ItĀ  uses a staged rotary action where the specimen is cut with the circular motion of the handwheel. The cutting procedure is done with a knife or disposable blade placed within the instrument.
  3. It has various parts: knife holder base, knife holder, microtome body,Ā cassette clamp or block holder, advancement handwheel, coarse handwheel, micron adjustment knob, and safety lock.
  4. It has thick and large knives that produce fewer vibrations. A high-resolution motor is used that creates a high-precision, stable, and smooth automatic slicing system.
  5. The rotary microtome is used in various microbiological labs, including histopathology/anatomical pathology labs.
  6. The blades of the rotary microtome should be handled with care. Always use cut-resistant gloves to remove the blades. Before cleaning, remove the blades from the holder and lock the rotary machine.

References

  1. Tosney, K. W., & Landmesser, L. T. (1986).Ā Neurites and growth cones in the chick embryo. Enhanced tissue preservation and visualization of HRP-labeled subpopulations in serial 25-microns plastic sections cut on a rotary microtome.Ā Journal of Histochemistry & Cytochemistry,Ā 34(7), 953–957. https://doi.org/10.1177/34.7.3519758Ā 
  1. Sharma, R. (2022). Microtomy. Retrieved February 5, 2022, from Slideshare.net website: https://www.slideshare.net/candysharma777/microtomy-67236029
  2. Frimiano, E M S, N, C. N., A, V. D., Sales, A., Mendes, & A, N. A. (2022). Histological study of the liver of the lizard Tropidurus torquatusWiedĀ 1820, (Squamata: Tropiduridae)(AU).Ā Braz. J. Morphol. Sci, 165–170. Retrieved from https://pesquisa.bvsalud.org/portal/resource/pt/lil-644137
  3. Muhd-Farouk, H., Amin-Safwan, A., Mahsol, H., & Ikhwanuddin, M. (2017). Histological Characteristics on the Testes of Mud Spiny Lobster, Panulirus polyphagus (Herbst, 1793).Ā Pakistan Journal of Biological Sciences: PJBS,Ā 20(7), 365-371. https://doi.org/10.3923/pjbs.2017.365.371
  4. Sy, J., & Ang, L.-C. (2018).Ā Microtomy: Cutting Formalin-Fixed, Paraffin-Embedded Sections.Ā Methods in Molecular Biology, 269–278. https://doi.org/10.1007/978-1-4939-8935-5_23

Additional information

Weight 108 lbs
Dimensions 69 × 59 × 50 cm
Model

Standard, Precision

Weight

32KG

Voltage

AC220V/110V

Frequency

50HZ/60Hz

Power

100W

Fuse

F1A L 250V

Certification

CE; ISO; 13485

Warranty

2 years Lifetime technical support

Customization

Acceptance

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